Thermo Scientific™ Bradford's reagent, ready-to-use

Thermo Scientific™ Bradford’s Reagent is a ready-to-use protein assay solution based on the Bradford method, utilizing Coomassie Brilliant Blue G-250 dye to detect and quantify protein concentrations in solution. The reagent binds primarily to basic and aromatic amino acids, leading to a measurable spectral shift in absorbance from 465 nm to 595 nm upon protein binding.

This reagent is supplied in a stable, ready-to-use liquid format, requiring no mixing, dilution, or reagent preparation, making it ideal for quick and consistent protein concentration measurements in microcentrifuge tubes, cuvettes, or microplate-based workflows.

Key Features and Benefits

• Ready-to-use formulation: No reagent mixing, pH adjustment, or dilution required
• Rapid assay protocol: Only 5-minute incubation before measurement
• Sensitive and versatile: Detects 1–10 μg/mL (micro assay) or 50–1400 μg/mL (standard assay)
• Minimal interference: Reducing sugars, thiols, and other reducing agents do not interfere
• Microwell compatible: Suitable for 96-well or 384-well microplate formats
• Cost-effective: Inexpensive, high-throughput protein quantification solution
• Broad compatibility: Effective for a wide range of purified or complex protein samples

Applications

• Total protein quantification in cell lysates, tissue extracts, or purified protein solutions
• Protein normalization for Western blotting, ELISA, and other downstream analyses
• High-throughput protein screening in microplate assays
• Biochemical and molecular biology research workflows

Assay Protocol (Simplified)

1. Mix sample or protein standard (e.g., BSA) with Bradford reagent in appropriate vessel
2. Incubate for 5 minutes at room temperature
3. Measure absorbance at 595 nm using spectrophotometer or plate reader
4. Quantify using a standard curve generated from known protein concentrations

Thermo Scientific™ Bradford’s Reagent offers a convenient, fast, and highly reliable solution for dye-based protein quantification using the time-tested Bradford method. Its ready-to-use format, broad assay range, and minimal interference from reducing agents make it ideal for routine protein quantitation across life sciences, biochemistry, and proteomics labs.