Thermo Scientific™ 3,3',5,5'-Tetramethylbenzidine dihydrochloride hydrate 98+%

Thermo Scientific™ 3,3',5,5'-Tetramethylbenzidine dihydrochloride hydrate (TMB-HCl hydrate) 98+% is a high-purity chromogenic substrate commonly used in enzymatic assays, biochemical analysis, and diagnostic applications. This reagent is a stable, water-soluble form of TMB, an oxidation-sensitive molecule that serves as a substrate for peroxidase-based detection systems such as ELISA (Enzyme-Linked Immunosorbent Assays) and Western blot assays.

TMB-HCl hydrate is particularly useful in colorimetric assays, where it produces a blue-colored oxidized product upon reaction with peroxidases (e.g., horseradish peroxidase, HRP). The reaction can be stopped with an acid, yielding a yellow-colored solution that absorbs at 450 nm, making it ideal for spectrophotometric quantification.

Key Features & Benefits

✔ High Purity (≥98.0%) – Ensures reliable, reproducible results in enzymatic assays.

✔ Water-Soluble Form – Improved stability and solubility compared to free TMB.

✔ Chromogenic Substrate for HRP – Produces a blue color upon peroxidase oxidation, which shifts to yellow upon acid stop reaction.

✔ Wide Applications – Used in ELISA, Western blotting, immunoassays, and biochemical research.

✔ Stable at Refrigerated Temperatures – Storage at 2-8°C ensures long-term stability.

Chemical & Physical Properties

• Molecular Formula: C₁₆H₂₀N₂·2HCl·xH₂O
• Molecular Weight: 313.27 g/mol (anhydrous form)
• Appearance: Off-white powder
• Melting Point: >300°C
• Solubility: Water-soluble
• Storage Temperature: Refrigerated (2-8°C)

Applications

🔹 Enzyme-Linked Immunosorbent Assay (ELISA) – Acts as a chromogenic substrate for HRP-based detection.

🔹 Western Blot Assays – Enhances visualization of peroxidase-linked antibody reactions.

🔹 Diagnostic Kits – Used in medical and research diagnostics for pathogen detection.

🔹 Biochemical Research – Investigates peroxidase activity and oxidation-reduction reactions.

🔹 Colorimetric Assays – Used for the quantitative determination of peroxidase activity.

Specifications & Test Results

Safety & Handling

⚠ Hazard Class: UN 2811 (Toxic Substance, ADR 6.1, III)

⚠ Handling Precautions:

• Use personal protective equipment (PPE) – gloves, lab coat, and eye protection.
• Avoid inhalation and skin contact – handle in a fume hood when possible.
• Dispose of waste according to local regulations.

Ordering Information

📦 Shipping Condition: Ambient temperature

❄ Storage Condition: Refrigerated (2-8°C)

Thermo Scientific™ 3,3',5,5'-Tetramethylbenzidine dihydrochloride hydrate (TMB-HCl hydrate) is a high-purity, stable, and water-soluble chromogenic substrate, widely used in ELISA, Western blotting, and biochemical research. With its high sensitivity, stability, and ease of use, it is an ideal reagent for HRP-based detection systems in diagnostic and research applications.