Thermo Scientific™ 2,2-Diphenyl-1-picrylhydrazyl (DPPH) ≥95% free radical

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Formula: C₁₈H₁₂N₅O₆
CAS Number: 1898-66-4

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Specifications:
Application Free Radical Analysis
Storage Temperature 2-8°C
Product Type Laboratory Chemical Forms Solid
Product Brand Thermo Fisher Scientific™
Product Grade Analytical grade Formula C₁₈H₁₂N₅O₆

2,2-Diphenyl-1-picrylhydrazyl (DPPH) is a highly stable free radical commonly used as a sensitive colorimetric reagent in antioxidant detection and other free radical-related studies. It is particularly valuable for monitoring chemical reactions involving radicals and serves as a reference for electron paramagnetic resonance (EPR) studies.

Key Features:

  1. Applications:
    • Antioxidant Assays: Used as a general detector for free radical scavenging activity.
    • Chemical Reaction Monitoring: Monitors reactions involving radicals.
    • EPR Studies: Standard for determining the position and intensity of EPR signals.
    • Quantitative Determination: Measures aliphatic and aromatic thiols using indirect spectroscopy.
    • Tocopherol Detection: Facilitates the photometric quantification of tocopherol.
  2. Physical Properties:
    • Appearance: Green to black powder.
    • Molecular Formula: C₁₈H₁₂N₅O₆.
    • Molecular Weight: 394.32 g/mol.
  3. Chemical Stability:
    • A stable free radical with consistent behavior under standard laboratory conditions.
  4. Pack Sizes:
    • 250 mg: Catalog Number 44150.MD.
    • 1 g: Catalog Number 44150.03.

Specifications:

PropertyDetails
Purity≥95%
AppearanceGreen to black powder
Molecular FormulaC₁₈H₁₂N₅O₆
Molecular Weight394.32 g/mol
CAS Number1898-66-4
MDL NumberMFCD00007231
EINECS Number217-591-8
Merck Index Number14,3330

Applications in Detail:

  1. Antioxidant Detection:
    • Reacts with antioxidants to reduce the DPPH radical, causing a color change from purple to yellow, measurable spectrophotometrically at 517 nm.
  2. Radical Reaction Monitoring:
    • Tracks the generation and scavenging of free radicals in chemical systems.
  3. EPR Standards:
    • Provides a reference for the calibration of electron paramagnetic resonance equipment.
  4. Quantification of Thiols:
    • Utilized for indirect spectroscopic measurements of thiol compounds.
  5. Photometric Tocopherol Determination:
    • Allows precise quantification of tocopherol (Vitamin E) concentrations.

Advantages:

FeatureBenefit
High StabilityAllows for reproducible and reliable measurements.
Colorimetric AssayEnables easy visual and spectrophotometric detection of radical activity.
Versatile ApplicationsSuitable for antioxidant detection, EPR, and radical reaction monitoring.

Storage and Handling:

  • Store in a cool, dry place to maintain stability.
  • Handle with care, following proper laboratory safety protocols.

Available Pack Sizes:

Catalog NumberPack SizeContainer Type
44150.MD250 mgSealed bottle
44150.031 gSealed bottle

  • Pack Size:   250mg 1g
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