Thermo Scientific™ 2,2-Diphenyl-1-picrylhydrazyl (DPPH) ≥95% free radical
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Formula: C₁₈H₁₂N₅O₆
CAS Number: 1898-66-4
Specifications:
Application | Free Radical Analysis | ||
Storage Temperature | 2-8°C | ||
Product Type | Laboratory Chemical | Forms | Solid |
Product Brand | Thermo Fisher Scientific™ | ||
Product Grade | Analytical grade | Formula | C₁₈H₁₂N₅O₆ |
2,2-Diphenyl-1-picrylhydrazyl (DPPH) is a highly stable free radical commonly used as a sensitive colorimetric reagent in antioxidant detection and other free radical-related studies. It is particularly valuable for monitoring chemical reactions involving radicals and serves as a reference for electron paramagnetic resonance (EPR) studies.
Key Features:
- Applications:
- Antioxidant Assays: Used as a general detector for free radical scavenging activity.
- Chemical Reaction Monitoring: Monitors reactions involving radicals.
- EPR Studies: Standard for determining the position and intensity of EPR signals.
- Quantitative Determination: Measures aliphatic and aromatic thiols using indirect spectroscopy.
- Tocopherol Detection: Facilitates the photometric quantification of tocopherol.
- Physical Properties:
- Appearance: Green to black powder.
- Molecular Formula: C₁₈H₁₂N₅O₆.
- Molecular Weight: 394.32 g/mol.
- Chemical Stability:
- A stable free radical with consistent behavior under standard laboratory conditions.
- Pack Sizes:
- 250 mg: Catalog Number 44150.MD.
- 1 g: Catalog Number 44150.03.
Specifications:
Property | Details |
---|---|
Purity | ≥95% |
Appearance | Green to black powder |
Molecular Formula | C₁₈H₁₂N₅O₆ |
Molecular Weight | 394.32 g/mol |
CAS Number | 1898-66-4 |
MDL Number | MFCD00007231 |
EINECS Number | 217-591-8 |
Merck Index Number | 14,3330 |
Applications in Detail:
- Antioxidant Detection:
- Reacts with antioxidants to reduce the DPPH radical, causing a color change from purple to yellow, measurable spectrophotometrically at 517 nm.
- Radical Reaction Monitoring:
- Tracks the generation and scavenging of free radicals in chemical systems.
- EPR Standards:
- Provides a reference for the calibration of electron paramagnetic resonance equipment.
- Quantification of Thiols:
- Utilized for indirect spectroscopic measurements of thiol compounds.
- Photometric Tocopherol Determination:
- Allows precise quantification of tocopherol (Vitamin E) concentrations.
Advantages:
Feature | Benefit |
---|---|
High Stability | Allows for reproducible and reliable measurements. |
Colorimetric Assay | Enables easy visual and spectrophotometric detection of radical activity. |
Versatile Applications | Suitable for antioxidant detection, EPR, and radical reaction monitoring. |
Storage and Handling:
- Store in a cool, dry place to maintain stability.
- Handle with care, following proper laboratory safety protocols.
Available Pack Sizes:
Catalog Number | Pack Size | Container Type |
---|---|---|
44150.MD | 250 mg | Sealed bottle |
44150.03 | 1 g | Sealed bottle |
- Pack Size: 250mg 1g