SuperSignal™ West Pico PLUS Chemiluminescent Substrate

The Thermo Scientific™ SuperSignal™ West Pico PLUS Chemiluminescent Substrate is an advanced enhanced chemiluminescent (ECL) detection reagent designed for picogram to high femtogram-level protein detection in western blot applications. This substrate delivers exceptional signal intensity and extended duration, enabling multiple exposures and high-quality imaging for publication-ready results.

Its broad compatibility with nitrocellulose and PVDF membranes, various blocking reagents, and wide antibody dilution ranges makes it a reliable choice for routine and high-sensitivity immunoblotting.

Key Features & Benefits

• High Sensitivity – Detect proteins at picogram to high femtogram levels for maximum clarity.
• Extended Signal Duration – Stable chemiluminescent signal for 6 to 24 hours, allowing multiple exposures.
• Excellent Stability – 8-hour working solution stability; 1-year kit stability at room temperature.
• Broad Compatibility – Works with both nitrocellulose and PVDF membranes and multiple blocking systems.
• Flexible Antibody Dilutions – Primary: 1:1000–1:5000 (0.2–1.0 μg/mL); Secondary: 1:20,000–100,000 (10–50 ng/mL).
• Easy Integration – Compatible with existing HRP-based western blotting workflows.

Applications

• Western Blotting – High-sensitivity detection of HRP-conjugated antibodies.
• Protein Expression Analysis – Ideal for detecting low-abundance proteins.
• Multiple Exposure Imaging – Capture high-resolution images over extended periods.

Technical Specifications

Ordering Information

Storage: Store at room temperature.

Regulatory Status: For Research Use Only – Not for use in diagnostic procedures.

The Thermo Scientific™ SuperSignal™ West Pico PLUS Chemiluminescent Substrate offers a dependable, high-sensitivity solution for western blot detection, enabling researchers to achieve precise, reproducible, and publication-quality results. Its combination of long-lasting signal, broad compatibility, and excellent sensitivity makes it an ideal choice for both routine and advanced protein detection workflows. Whether working with abundant or low-expression targets, this substrate ensures accurate visualization with minimal background and maximum clarity.