Thermo Scientific™ Oxoid™ Neomycin Selective Supplement, Freeze-Dried,10 x 150 mg

The Thermo Scientific™ Oxoid™ Neomycin Selective Supplement is a freeze-dried reagent specifically formulated to enhance the selective cultivation of clostridia and other anaerobic microorganisms. It works by incorporating neomycin, an antibiotic that inhibits the growth of non-target organisms, thereby promoting the proliferation of specific anaerobes in mixed cultures. This makes it an essential tool for microbiologists working with anaerobic bacteria in research, clinical diagnostics, and industrial applications.

Key Features

1. Selective Growth:
   • Designed to suppress unwanted bacterial flora while allowing the growth of targeted anaerobic microorganisms, such as clostridia.
2. Freeze-Dried Form:
   • Convenient for long-term storage and easy reconstitution into culture media.
3. High Compatibility:
   • Optimized for use with:
     • Blood Agar
     • Wilkins-Chalgren Anaerobe Agar (Part No. CM0619B)
     • Columbia Blood Agar (Part No. CM0331B)
4. Standardized Yield:
   • Each 150mg vial is calibrated to supplement 2 liters of culture medium, ensuring consistent results across batches.

Use Cases

1. Clinical Microbiology:

• Isolation of Anaerobic Pathogens:
  • Facilitates the identification and growth of anaerobic pathogens, such as Clostridium difficile, Clostridium perfringens, or Bacteroides spp., from patient specimens in clinical laboratories.
  • Commonly used in diagnostic workflows to support the management of infections like gas gangrene, food poisoning, or pseudomembranous colitis.

2. Food Microbiology:

• Detection of Contaminants:
  • Applied in food testing laboratories to isolate clostridia that may cause spoilage or pose food safety concerns, such as Clostridium botulinum in canned goods or Clostridium sporogenes in processed foods.
• Quality Control:
  • Used in anaerobic challenge testing to evaluate the shelf life of vacuum-sealed or canned food products.

3. Pharmaceutical Industry:

• Probiotic Research:
  • Supports research into anaerobic bacteria, such as Clostridium butyricum, used in probiotic formulations for gut health.
• Drug Development:
  • Aids in studying the susceptibility of anaerobes to new antimicrobial agents.

4. Environmental Microbiology:

• Soil and Wastewater Studies:
  • Utilized to isolate anaerobic microorganisms involved in biodegradation processes or nitrogen cycling in soil and water samples.
• Biogas Research:
  • Supports the identification of anaerobic bacteria that contribute to methane production in biogas reactors.

5. Academic and Research Laboratories:

• Microbial Taxonomy:
  • Assists researchers in studying the taxonomy, physiology, and genetics of clostridia and related anaerobes.
• Model Systems:
  • Used in experimental setups to simulate anaerobic environments for studying microbial interactions and ecosystems.

Preparation and Application

1. Reconstitution:
   • Dissolve the contents of one 150mg vial in 2 liters of pre-prepared culture medium (e.g., Blood Agar or Wilkins-Chalgren Anaerobe Agar).
   • Autoclave if required, or add aseptically to sterile medium.
2. Usage:
   • Pour the supplemented medium into Petri dishes or tubes for solid or liquid cultures.
   • Incubate plates or tubes under anaerobic conditions (e.g., anaerobic jars or chambers).

Specifications

Advantages

1. Efficient Target Isolation: Reduces contamination from non-target organisms, streamlining anaerobic culturing processes.
2. Reliable Performance: Standardized supplement ensures consistent, reproducible results.
3. Broad Applicability: Ideal for clinical, industrial, and research settings requiring anaerobic bacterial isolation.

For laboratories and industries requiring robust anaerobic culturing capabilities, the Oxoid Neomycin Selective Supplement is an indispensable resource. Contact your supplier for pricing and availability or request a quote for bulk purchases.