Human Luteinizing Hormone (LH)ELISA Kit

The Human Luteinizing Hormone (LH) ELISA Kit is a highly sensitive and specific assay designed for the quantitative detection of LH levels in human serum, plasma, urine, culture media, and other biological fluids. This kit is ideal for research on reproductive health, endocrine function, and related disorders.

Sample Preparation

1. Serum Preparation

1. Collect whole blood and allow it to clot at room temperature for 10–20 minutes.
2. Centrifuge at 2,000–3,000 rpm for 20 minutes to separate the serum.
3. Re-centrifuge if precipitates appear during storage.

2. Plasma Preparation

1. Collect whole blood into tubes containing anticoagulants (e.g., EDTA or citrate).
2. Incubate at room temperature for 10–20 minutes.
3. Centrifuge at 2,000–3,000 rpm for 20 minutes to obtain plasma.
4. Re-centrifuge if necessary.

3. Urine Samples

1. Collect urine in aseptic tubes.
2. Centrifuge at 2,000–3,000 rpm for 20 minutes and carefully collect the supernatant.
3. Re-centrifuge if precipitates form during storage.

4. Cell Samples

1. For cell secretions, collect culture supernatants in aseptic tubes and centrifuge at 2,000–3,000 rpm for 20 minutes.
2. For intracellular components:
   • Dilute cells in PBS (pH 7.2–7.4) at 1×10⁷/mL.
   • Disrupt cells via repeated freeze-thaw cycles.
   • Centrifuge at 2,000–3,000 rpm for 20 minutes and collect the supernatant.

5. Tissue Samples

1. Cut and weigh tissue samples, then freeze in liquid nitrogen and store at -80°C.
2. Homogenize samples in PBS (pH 7.4) at 4°C.
3. Centrifuge at 2,000–3,000 rpm for 20 minutes and collect the supernatant.

Important Notes

1. Perform ELISA assays as soon as possible after sample collection.
2. Store samples at -20°C if immediate testing is not feasible.
3. Avoid repeated freeze-thaw cycles to preserve sample integrity.
4. Do not use samples containing sodium azide (NaN₃) as it inhibits HRP activity.

Applications

• Reproductive Health Research:
  • Study LH levels in ovulation, menstrual cycles, and fertility.
• Endocrine Studies:
  • Explore LH’s role in hormonal balance and its interaction with other gonadotropins like FSH.
• Clinical Diagnostics Research:
  • Investigate LH as a biomarker for menopause, infertility, and hypogonadism.
• Cancer Research:
  • Assess LH levels in hormone-sensitive cancers.
• Therapeutic Development:
  • Monitor LH as a target in treatments for reproductive disorders.

Advantages

1. High Sensitivity and Specificity:
   • Detects low levels of LH with minimal cross-reactivity or interference.
2. Wide Sample Compatibility:
   • Validated for serum, plasma, urine, cell culture supernatants, and tissue homogenates.
3. Ease of Use:
   • Includes pre-coated plates and ready-to-use reagents.
4. Reproducibility:
   • Provides consistent and reliable results across multiple experiments.

Storage and Stability

• Store all kit components at 2–8°C.
• Avoid repeated freeze-thaw cycles to ensure assay reliability.

The Human Luteinizing Hormone (LH) ELISA Kit is an essential tool for researchers investigating LH’s role in reproductive health, endocrine regulation, and related diseases. Its precision, user-friendly design, and broad compatibility make it indispensable for various clinical and experimental applications.