Human N-Terminal Pro-Brain Natriuretic Peptide (Nt-Probnp) Elisa Kit

The Human NT-ProBNP ELISA Kit is a sensitive and specific assay designed for the quantitative detection of NT-ProBNP levels in human serum, plasma, culture media, and other biological fluids. This kit is essential for research into heart failure, cardiovascular health, and related disorders.

Purpose

• Quantification of NT-ProBNP Levels:
  • Detect and measure NT-ProBNP concentrations in biological samples for research into cardiovascular health and disease.
• Sample Compatibility:
  • Suitable for human serum, plasma, and other biological fluids.

Principle

The assay utilizes a Sandwich-ELISA technique to ensure accuracy and specificity. Below is the process:

1. Pre-Coated Plate:
   • Microelisa stripplate wells are pre-coated with an antibody specific to NT-ProBNP.
2. Sample and Standard Addition:
   • Standards and samples are added to the wells, where NT-ProBNP in the sample binds to the immobilized antibody.
3. HRP-Conjugated Detection:
   • A Horseradish Peroxidase (HRP)-conjugated antibody specific to NT-ProBNP is added, forming a sandwich complex.
4. Washing Step:
   • Free and unbound components are washed away to minimize background interference.
5. Substrate Reaction:
   • TMB substrate solution reacts with HRP, producing a blue color in wells containing NT-ProBNP.
6. Stop Reaction and Measurement:
   • A stop solution is added, changing the color from blue to yellow. Optical density (OD) is measured at 450 nm using a spectrophotometer.
7. Quantification:
   • NT-ProBNP concentrations are calculated by comparing OD values to a standard curve.

Key Features

1. High Sensitivity and Specificity:
   • Accurately detects low levels of NT-ProBNP with minimal cross-reactivity.
2. Wide Sample Compatibility:
   • Validated for serum, plasma, culture media, and other biological fluids.
3. Quantitative Results:
   • Provides precise measurement of NT-ProBNP concentrations using a standard curve.
4. Ease of Use:
   • Includes pre-coated plates and ready-to-use reagents for a streamlined workflow.
5. Reproducibility:
   • Ensures consistent and reliable results across multiple experiments.

Kit Components

• Pre-coated Microelisa stripplate.
• NT-ProBNP standards for standard curve generation.
• HRP-conjugated NT-ProBNP detection antibody.
• TMB substrate solution.
• Stop solution.
• Wash buffer.
• Sample diluent.
• Instruction manual.

Applications

• Cardiovascular Research:
  • Study NT-ProBNP as a biomarker for heart failure and myocardial stress.
• Heart Failure Diagnosis:
  • Investigate NT-ProBNP levels in patients with acute or chronic heart failure.
• Therapeutic Development:
  • Evaluate the effectiveness of drugs targeting heart failure or cardiovascular health.
• Clinical Diagnostics Research:
  • Support diagnostics for cardiovascular diseases and risk assessment.
• Epidemiological Studies:
  • Monitor NT-ProBNP as a marker in population-level cardiovascular research.

Advantages

1. High Accuracy:
   • Provides precise and reproducible quantification of NT-ProBNP.
2. Convenience:
   • Ready-to-use reagents simplify preparation and assay execution.
3. Versatility:
   • Compatible with various biological sample types.
4. Reliable:
   • Delivers consistent results for robust data interpretation.

Assay Protocol Summary

1. Add standards or samples to the pre-coated wells.
2. Incubate with HRP-conjugated NT-ProBNP antibody.
3. Wash to remove unbound components.
4. Add TMB substrate to develop color.
5. Stop the reaction and measure OD at 450 nm.
6. Calculate NT-ProBNP concentrations using the standard curve.

Storage and Stability

• Store all kit components at 2–8°C to maintain reagent integrity.
• Avoid repeated freeze-thaw cycles to ensure assay reliability.

The Human NT-ProBNP ELISA Kit is an essential tool for researchers studying heart failure, cardiovascular health, and related disorders. Its precision, ease of use, and broad compatibility make it indispensable for a variety of clinical and experimental applications.