Human Hepatitis B Virus Surface Antigen, Hbsag Elisa Kit

The Human Hepatitis B Virus Surface Antigen (HBsAg) ELISA Kit is a highly sensitive and specific qualitative assay designed for the detection of HBsAg in human serum, plasma, culture media, and other biological fluids. This kit is essential for research into hepatitis B virus (HBV) infections, immune response, and related conditions.

Purpose

• Qualitative Detection of HBsAg:
  • Identifies the presence of HBsAg in biological samples for HBV research and diagnostics.
• Sample Compatibility:
  • Suitable for human serum, plasma, and other biological fluids.

Principle

The assay employs a qualitative enzyme immunoassay technique for the detection of HBsAg. Below is the workflow:

1. Pre-Coated Plate:
   • Microplate wells are pre-coated with an antibody specific to HBsAg, forming a solid-phase antibody.
2. Sample Addition:
   • Samples are added to the wells, where HBsAg in the sample binds to the immobilized antibody.
3. HRP-Conjugated Detection:
   • A Horseradish Peroxidase (HRP)-conjugated antibody specific for HBsAg is added, forming an antibody-antigen-enzyme labeled antibody complex.
4. Washing Step:
   • Free and unbound components are washed away to minimize background interference.
5. Substrate Reaction:
   • TMB substrate solution reacts with HRP, producing a blue color in wells with HBsAg.
6. Stop Reaction and Measurement:
   • A stop solution is added, changing the color from blue to yellow. Optical density (OD) is measured at 450 nm using a spectrophotometer.
7. Qualitative Determination:
   • The presence of HBsAg is determined by comparing OD values with the CUTOFF value.

Key Features

1. High Sensitivity and Specificity:
   • Qualitatively detects HBsAg with minimal cross-reactivity or interference.
2. Wide Sample Compatibility:
   • Suitable for serum, plasma, culture media, and other biological fluids.
3. User-Friendly:
   • Includes pre-coated plates and ready-to-use reagents for straightforward workflows.
4. Reproducibility:
   • Ensures consistent and reliable results across multiple experiments.
5. Qualitative Analysis:
   • Provides a clear determination of HBsAg presence compared to a cutoff value.

Kit Components

• Pre-coated Microplate with solid-phase antibody.
• HRP-conjugated HBsAg detection antibody.
• TMB substrate solution.
• Stop solution.
• Wash buffer.
• Sample diluent.
• Instruction manual.

Applications

• Hepatitis B Virus Research:
  • Study HBV infections and immune responses to the virus.
• Epidemiological Studies:
  • Investigate the prevalence of HBV in populations.
• Clinical Diagnostics Research:
  • Support diagnostics for HBV-related conditions.
• Vaccine Development:
  • Assess immune responses to HBV vaccines.

Advantages

1. High Accuracy:
   • Provides precise and reproducible detection of HBsAg.
2. Convenience:
   • Ready-to-use reagents simplify preparation and assay execution.
3. Versatility:
   • Compatible with various biological sample types.
4. Reliable:
   • Delivers consistent results for robust data interpretation.

Assay Protocol Summary

1. Add samples to the pre-coated wells.
2. Incubate with HRP-conjugated HBsAg antibody.
3. Wash to remove unbound components.
4. Add TMB substrate to develop color.
5. Stop the reaction and measure OD at 450 nm.
6. Determine the presence of HBsAg by comparing OD values to the cutoff value.

Storage and Stability

• Store all kit components at 2–8°C to maintain reagent integrity.
• Avoid repeated freeze-thaw cycles to ensure assay reliability.

The Human Hepatitis B Virus Surface Antigen (HBsAg) ELISA Kit is an essential tool for detecting HBsAg in biological samples. Its sensitivity, specificity, and user-friendly design make it invaluable for research into HBV infections, epidemiology, and vaccine development.