Sigma-Aldrich α-Cyano-4-hydroxycinnamic acid matrix substance for MALDI-MS, ≥99.0% (HPLC)

Sigma-Aldrich α-Cyano-4-hydroxycinnamic acid (α-CHCA) is a high-purity matrix substance tailored for matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS). With a purity of ≥99.0% (HPLC), this product is designed for consistent, reproducible results in applications requiring precise ionization of proteins, peptides, and other analytes.

Available in 1g and 250mg quantities, this α-CHCA formulation supports a wide range of research needs, from routine analyses to advanced proteomics and metabolomics applications.

Key Features:

• High Purity (≥99.0% by HPLC): Ensures minimal background interference and maximized signal intensity for high-resolution mass spectrometry analysis.
• MALDI-Specific: Functions as a matrix for effective energy transfer during desorption/ionization in MALDI-MS workflows.
• Reliable and Reproducible: Offers consistent performance for routine and advanced analytical applications.
• Flexible Packaging: Available in both 250mg and 1g sizes to meet varying research scales and throughput requirements.

Specifications:

• Chemical Name: α-Cyano-4-hydroxycinnamic acid
• Chemical Formula: C10H7NO3
• Molecular Weight: 189.17 g/mol
• Purity: ≥99.0% (HPLC)
• Physical Form: Crystalline powder
• Solubility: Soluble in organic solvents such as acetonitrile and methanol.

Applications:

1. Proteomics:
   • Aids in the ionization of peptides and proteins for high-precision identification and quantitation.
   • Frequently used in tryptic digest analyses for protein characterization.
2. Metabolomics:
   • Supports the analysis of small metabolites in complex biological samples.
   • Enhances signal clarity for low-abundance analytes.
3. Biomarker Discovery:
   • Facilitates detection of disease-related biomarkers in clinical and translational research.
4. Pharmaceutical Research:
   • Applied in drug discovery and development to identify small drug molecules and their metabolites.
5. Peptide Mass Fingerprinting (PMF):
   • Ideal for rapid and accurate peptide identification.

Benefits:

• High Sensitivity: Detects trace-level analytes with strong signal intensities.
• Reduced Background Interference: The high purity ensures cleaner spectra and improved signal-to-noise ratios.
• Versatile Compatibility: Suitable for a wide range of analytes, including peptides, proteins, small molecules, and nucleic acids.
• Ease of Use: Dissolves readily in MALDI solvents for rapid preparation of matrix-analyte mixtures.

Storage and Handling:

• Storage Conditions: Store in a cool, dry place, preferably at 2-8°C, away from light and moisture.
• Handling: Use appropriate safety measures, including gloves and eye protection. Avoid inhalation or contact with skin and eyes.

Packaging Options:

1. 1g: Ideal for labs requiring larger quantities for high-throughput analyses.
2. 250mg: Suitable for routine use or labs with lower sample volumes.

Sigma-Aldrich α-Cyano-4-hydroxycinnamic acid (α-CHCA) with ≥99.0% purity is a gold standard matrix for MALDI-MS applications. Its high performance, coupled with flexible packaging, makes it a versatile choice for proteomics, metabolomics, biomarker discovery, and pharmaceutical research. Whether analyzing low-abundance biomarkers or conducting routine mass spectrometry, this α-CHCA formulation ensures reliable, high-quality results.