Sigma-Aldrich Trizma® hydrochloride solution, pH 7.4, BioPerformance Certified, 1 M, suitable for cell culture

The Sigma-Aldrich Trizma® Hydrochloride Solution is a 1 M, pre-mixed Tris buffer solution, designed for molecular biology and cell culture applications. It is BioPerformance Certified, ensuring high purity and reproducibility for sensitive biological and biochemical experiments. The solution has a pH of 7.4, which falls within the optimal range (7.0–9.0) for various laboratory applications.

Key Features & Benefits

1. Ready-to-Use Buffer Solution

• Pre-mixed Trizma Base and Trizma HCl, eliminating the need for manual pH adjustment or preparation.
• Maintains highly accurate pH (± 0.1 pH units), ensuring consistency in experiments.

2. High Purity – BioPerformance Certified

• DNase, RNase, and Protease-free, making it ideal for nucleic acid and protein research.
• Low endotoxin levels, making it suitable for mammalian cell culture applications.
• ≤5 ppm heavy metals (as Pb), ensuring minimal contamination.

3. Versatile pH Range (7.0–9.0)

• The pH of 7.4 is widely used in cell culture, enzymatic reactions, and biochemical assays.
• Works as a buffer for protein stability, nucleic acid preservation, and various molecular biology workflows.

Specifications

Applications

1. Cell Culture & Biochemical Assays

• Maintains physiological pH for mammalian cell culture experiments.
• Used in enzymatic reactions that require a stable buffering environment.
• Helps in the stabilization of proteins and nucleic acids.

2. Molecular Biology & Genomics

• RNA & DNA Research: Prevents degradation due to contamination (RNase-free, DNase-free).
• Used in the preparation of lysis buffers for cell or tissue extraction.
• A key component in wash buffers and nucleic acid isolation protocols.

3. Protein Chemistry & Structural Biology

• Utilized in protein purification and Western blotting protocols.
• Essential for electrophoresis buffers to maintain protein structure and activity.
• Used in biophysical and biochemical characterization of proteins.

4. Synthetic & Nanomaterial Applications

• Acts as a buffer in nanoparticle and microreactor synthesis.
• Used in dissolving cadmium- or sulfide-loaded microreactors for CdS synthesis.

Pack Sizes & Catalog Numbers

Storage & Handling

• Store at room temperature (RT).
• Avoid contamination to maintain purity (DNase, RNase, and protease-free certification).
• Use sterile pipettes and clean handling techniques for molecular biology and cell culture applications.

Summary

The Sigma-Aldrich Trizma® Hydrochloride Solution (1 M, pH 7.4) is a high-purity buffer solution, optimized for molecular biology, cell culture, and protein research. Its DNase-, RNase-, and protease-free formulation makes it an ideal choice for sensitive biochemical applications, while its pre-mixed, pH-stable formulation simplifies laboratory workflows.