Sigma-Aldrich Protein Standard Mix 15 - 600 kDa, for size exclusion chromatography

The Protein Standard Mix 15–600 kDa is a lyophilized mixture of molecular weight markers designed for calibration and performance monitoring of size exclusion chromatography (SEC) columns. With molecular weight standards ranging from 15 kDa to 600 kDa, this mix is essential for ensuring reliable and reproducible SEC results in various applications, including protein separation, pharmaceutical research, and clinical studies.

Key Features:

1. Wide Molecular Weight Range:
   • Standards cover molecular weights from 15 kDa to 600 kDa, suitable for diverse SEC applications.
2. High-Quality Calibration:
   • Ensures the accuracy and efficiency of SEC column selectivity and separation.
3. Versatile Application:
   • Compatible with gel permeation chromatography (GPC) and size exclusion chromatography (SEC).
4. Lyophilized for Stability:
   • Ready-to-use solid form ensures extended shelf life and ease of storage.
5. Broad Use Cases:
   • Supports research in clinical, food and beverage, and pharmaceutical applications.

Specifications:

Applications:

1. SEC Column Calibration:
   • Evaluates the selectivity and separation efficiency of SEC columns, ensuring consistent performance.
2. Protein Molecular Weight Estimation:
   • Determines the molecular masses of proteins and protein complexes.
3. Oligomerization Studies:
   • Used for separation and analysis of oligomerized and nitrated proteins.
4. Pharmaceutical Protein Analysis:
   • Supports the development and characterization of therapeutic proteins, such as recombinant proteins and antibody-based therapeutics.
5. Biochemical Research:
   • Enables non-invasive analysis of protein behavior in vitro, including binding interactions and structural studies.

Components:

• Thyroglobulin (bovine): ~670,000 Da
• γ-Globulins (bovine): ~150,000 Da
• Ovalbumin: ~44,300 Da
• Ribonuclease A (Type I-A): ~13,700 Da
• p-Aminobenzoic acid (pABA): ~137 Da

Protocol Overview:

1. Prepare Solution:
   • Reconstitute the lyophilized protein mix in the recommended buffer.
2. Load onto SEC Column:
   • Inject the prepared solution into the SEC system to evaluate column performance.
3. Monitor Chromatogram:
   • Assess peaks corresponding to molecular weight standards to confirm column selectivity and efficiency.
4. Adjust Parameters:
   • Fine-tune flow rate, column size, or pore size based on calibration results.

Ordering Information:

Storage and Handling:

• Storage: Store at −20°C for long-term stability.
• Handling: Ensure samples are reconstituted in a compatible buffer for SEC analysis.

Advantages:

1. Reliable Calibration:
   • Ensures consistent and reproducible SEC column performance.
2. Broad Analytical Range:
   • Covers molecular weight markers suitable for a wide range of proteins and peptides.
3. High Purity Standards:
   • Lyophilized form ensures high stability and minimal contaminants.
4. Versatile Use Cases:
   • Applicable across various industries, including clinical, pharmaceutical, and biochemical research.
5. Convenient Storage:
   • Stable at −20°C, reducing the need for frequent replenishment.

Related Products:

• SEC Columns: Compatible with leading size exclusion chromatography systems.
• Molecular Biology Buffers: For reconstitution and sample preparation.
• Thermo Scientific™ Nuclease-Free Water: Ensures contamination-free buffer preparation.

Regulatory Notes:

• For Research Use Only: Not intended for diagnostic or therapeutic use.

The Protein Standard Mix 15–600 kDa is an indispensable tool for size exclusion chromatography calibration, delivering precision and reliability across a range of molecular weight analysis applications.