Invitrogen™ Streptavidin, Alkaline Phosphatase Conjugate, 0.5mL, 2 mg/mL

The Invitrogen™ Streptavidin, Alkaline Phosphatase (AP) Conjugate is a high-affinity biotin-binding reagent designed for robust signal detection in enzyme-based assays. It features streptavidin conjugated to alkaline phosphatase, enabling sensitive, substrate-based colorimetric or chemiluminescent detection of biotinylated antibodies, nucleic acids, or other biotin-labeled molecules.

Unlike avidin, streptavidin lacks glycosylation and has a near-neutral isoelectric point, which contributes to lower nonspecific background in detection workflows. This conjugate is particularly well-suited for applications that demand signal amplification and specificity.

Key Features & Benefits

• Strong Biotin Binding
  – Tetrameric streptavidin binds up to 4 biotin molecules with extremely high affinity (Kd ~10⁻¹⁵ M).
• Alkaline Phosphatase Enzyme Label
  – Enables substrate-based colorimetric or chemiluminescent detection in ELISA, blotting, and microscopy.
• Reduced Nonspecific Binding
  – Streptavidin's lack of carbohydrate groups results in lower background vs. avidin.
• Versatile Applications
  – Suitable for Western blotting, ELISA, immunohistochemistry (IHC), nucleic acid detection, and other biotin-based systems.
• Recombinant Streptavidin
  – Ensures consistency and batch-to-batch reliability.

Applications

• ELISA – Enzyme-linked detection of biotinylated probes or antibodies.
• Western Blotting – Colorimetric or chemiluminescent detection of biotinylated proteins.
• Immunohistochemistry (IHC) – Detection of biotinylated antibodies in tissue sections.
• Nucleic Acid Hybridization – Detect biotinylated DNA or RNA probes.
• Fluorescence Microscopy – When paired with AP substrates compatible with fluorescence imaging.

Specifications

Invitrogen™ Streptavidin, Alkaline Phosphatase Conjugate is a premium detection reagent offering exceptional biotin-binding capacity, enzymatic signal amplification, and minimal nonspecific background. It is ideal for biotin-based detection workflows in protein analysis, tissue imaging, and molecular diagnostics research, ensuring consistent and reproducible results in demanding applications.