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Sigma-Aldrich Laemmli Sample Buffer (2× concentrate) is a ready-to-use protein sample preparation buffer designed for SDS-PAGE applications following the Laemmli method. This formulation ensures optimal protein denaturation, reduction, and tracking during electrophoretic separation, enabling high-resolution analysis of protein molecular weight and purity.
Key Features
- SDS-Based Denaturing Buffer – Ensures proteins acquire a uniform negative charge for size-based separation
- Ready-to-Use 2× Concentrate – Mix 1:1 with your protein sample prior to loading
- Complete Sample Preparation – Contains reducing agent, tracking dye, and density enhancer for efficient sample handling
- Optimized for Laemmli SDS-PAGE System – Proven formulation for high-resolution protein electrophoresis
Composition
| Component | Concentration |
|---|---|
| SDS (Sodium Dodecyl Sulfate) | 4% |
| Glycerol | 20% |
| 2-Mercaptoethanol | 10% |
| Bromophenol Blue | 0.004% |
| Tris-HCl Buffer | 0.125 M, pH ~6.8 |
Biochemical Function
- Denaturation: SDS disrupts protein structure, while 2-mercaptoethanol reduces disulfide bonds
- Charge Equalization: Proteins acquire a consistent negative charge to enable separation by size
- Sample Visibility & Tracking: Bromophenol blue acts as a visual loading and migration marker
- Sample Layering: Glycerol ensures that samples settle efficiently in gel wells
Usage Instructions
- Recommended Usage: Mix 1 volume of sample with 1 volume of 2× Laemmli buffer
- Preparation: Heat the mixture at 95–100°C for 2–5 minutes before loading into the gel
Applications
- SDS-PAGE Analysis – For protein size estimation, purity analysis, and Western blotting
- Biochemical & Molecular Biology Research – Suitable for sample preparation in proteomics workflows
- Education & Training Labs – Standard buffer for protein electrophoresis protocols
Sigma-Aldrich Laemmli 2× Sample Buffer delivers a convenient and effective solution for denaturing and preparing protein samples for SDS-PAGE. With its proven formulation and ease of use, it is an essential reagent for consistent and high-quality protein electrophoresis results in research and diagnostic labs.
No resources are currently available for this product.
Sigma-Aldrich Laemmli Sample Buffer (2× concentrate) is a ready-to-use protein sample preparation buffer designed for SDS-PAGE applications following the Laemmli method. This formulation ensures optimal protein denaturation, reduction, and tracking during electrophoretic separation, enabling high-resolution analysis of protein molecular weight and purity.
Key Features
- SDS-Based Denaturing Buffer – Ensures proteins acquire a uniform negative charge for size-based separation
- Ready-to-Use 2× Concentrate – Mix 1:1 with your protein sample prior to loading
- Complete Sample Preparation – Contains reducing agent, tracking dye, and density enhancer for efficient sample handling
- Optimized for Laemmli SDS-PAGE System – Proven formulation for high-resolution protein electrophoresis
Composition
| Component | Concentration |
|---|---|
| SDS (Sodium Dodecyl Sulfate) | 4% |
| Glycerol | 20% |
| 2-Mercaptoethanol | 10% |
| Bromophenol Blue | 0.004% |
| Tris-HCl Buffer | 0.125 M, pH ~6.8 |
Biochemical Function
- Denaturation: SDS disrupts protein structure, while 2-mercaptoethanol reduces disulfide bonds
- Charge Equalization: Proteins acquire a consistent negative charge to enable separation by size
- Sample Visibility & Tracking: Bromophenol blue acts as a visual loading and migration marker
- Sample Layering: Glycerol ensures that samples settle efficiently in gel wells
Usage Instructions
- Recommended Usage: Mix 1 volume of sample with 1 volume of 2× Laemmli buffer
- Preparation: Heat the mixture at 95–100°C for 2–5 minutes before loading into the gel
Applications
- SDS-PAGE Analysis – For protein size estimation, purity analysis, and Western blotting
- Biochemical & Molecular Biology Research – Suitable for sample preparation in proteomics workflows
- Education & Training Labs – Standard buffer for protein electrophoresis protocols
Sigma-Aldrich Laemmli 2× Sample Buffer delivers a convenient and effective solution for denaturing and preparing protein samples for SDS-PAGE. With its proven formulation and ease of use, it is an essential reagent for consistent and high-quality protein electrophoresis results in research and diagnostic labs.
No resources are currently available for this product.