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Quantitative Sandwich ELISA for Detection of Testosterone in Rat Serum, Plasma, and Biological Samples
The Rat Testosterone ELISA Kit is a high-sensitivity, quantitative sandwich ELISA designed for the in vitro determination of testosterone (T) levels in rat serum, plasma, cell culture supernatants, and tissue samples. Using a microplate pre-coated with a testosterone-specific antibody, this assay delivers reliable and reproducible results, suitable for use in endocrine, reproductive biology, toxicology, and preclinical research.
This kit provides a sensitivity of 0.01 ng/mL, with a detection range of 0.1–3.8 ng/mL, making it ideal for precise analysis of testosterone fluctuations in physiological and experimental settings.
Key Features & Benefits
- ✅ High Sensitivity (0.01 ng/mL) – Enables detection of low testosterone levels in rat biological samples
- ✅ Wide Assay Range (0.1–3.8 ng/mL) – Accommodates both physiological and pathological hormone levels
- ✅ Validated Precision – Intra-assay CV <10%, Inter-assay CV <12%
- ✅ Multi-Sample Compatibility – Suitable for serum, plasma, urine, cell culture media, and tissue homogenates
- ✅ Complete Kit – Includes pre-coated microplate, standards, conjugates, buffers, and substrates
- ✅ Storage at 2–8 °C – Stable for up to 6 months under recommended conditions
- ✅ For Research Use Only (RUO) – Ideal for laboratory and preclinical applications
Kit Components (96 Tests)
| Component | Volume/Qty |
|---|---|
| MicroELISA strip plate (pre-coated) | 1 plate (8×12 wells) |
| Testosterone (T) Standard (4.5 ng/mL) | 0.5 mL × 1 bottle |
| Standard Diluent | 1.5 mL × 1 bottle |
| HRP-Conjugate Reagent | 6 mL × 1 bottle |
| Sample Diluent | 6 mL × 1 bottle |
| Chromogen Solution A (TMB A) | 6 mL × 1 bottle |
| Chromogen Solution B (TMB B) | 6 mL × 1 bottle |
| Stop Solution | 6 mL × 1 bottle |
| Wash Buffer (30× Concentrate) | 20 mL × 1 bottle |
| Closure Plate Membrane | 2 pieces |
| User Manual | 1 |
| Sealed Storage Bags | 1 |
Sample Types
- ✅ Rat serum
- ✅ Plasma (EDTA, citrate)
- ✅ Urine
- ✅ Cell culture supernatants or lysates
- ✅ Tissue homogenates
Assay Performance
| Attribute | Value |
|---|---|
| Assay Range | 0.1 – 3.8 ng/mL |
| Sensitivity | 0.01 ng/mL |
| Intra-assay CV | <10% |
| Inter-assay CV | <12% |
| Assay Time | ~2.5 hours |
| Reading | Absorbance at 450 nm |
Applications
🧬 Endocrinology Research – Monitor testosterone levels in hormonal studies
🧪 Reproductive Toxicology – Evaluate testicular function and hormone disruption
🧫 Preclinical Pharmacology – Assess drug effects on hormone levels in vivo
🧠 Behavioral Neuroscience – Study testosterone influence on animal behavior
🔬 Veterinary Research – Investigate hormonal biomarkers in animal models
The Rat Testosterone ELISA Kit (SL0668Ra) provides a sensitive, accurate, and easy-to-use platform for the quantification of testosterone in a wide range of biological fluids. Designed for research use only, this kit is ideal for scientists and labs conducting hormonal, toxicological, or reproductive studies in rat models.
No resources are currently available for this product.
Quantitative Sandwich ELISA for Detection of Testosterone in Rat Serum, Plasma, and Biological Samples
The Rat Testosterone ELISA Kit is a high-sensitivity, quantitative sandwich ELISA designed for the in vitro determination of testosterone (T) levels in rat serum, plasma, cell culture supernatants, and tissue samples. Using a microplate pre-coated with a testosterone-specific antibody, this assay delivers reliable and reproducible results, suitable for use in endocrine, reproductive biology, toxicology, and preclinical research.
This kit provides a sensitivity of 0.01 ng/mL, with a detection range of 0.1–3.8 ng/mL, making it ideal for precise analysis of testosterone fluctuations in physiological and experimental settings.
Key Features & Benefits
- ✅ High Sensitivity (0.01 ng/mL) – Enables detection of low testosterone levels in rat biological samples
- ✅ Wide Assay Range (0.1–3.8 ng/mL) – Accommodates both physiological and pathological hormone levels
- ✅ Validated Precision – Intra-assay CV <10%, Inter-assay CV <12%
- ✅ Multi-Sample Compatibility – Suitable for serum, plasma, urine, cell culture media, and tissue homogenates
- ✅ Complete Kit – Includes pre-coated microplate, standards, conjugates, buffers, and substrates
- ✅ Storage at 2–8 °C – Stable for up to 6 months under recommended conditions
- ✅ For Research Use Only (RUO) – Ideal for laboratory and preclinical applications
Kit Components (96 Tests)
| Component | Volume/Qty |
|---|---|
| MicroELISA strip plate (pre-coated) | 1 plate (8×12 wells) |
| Testosterone (T) Standard (4.5 ng/mL) | 0.5 mL × 1 bottle |
| Standard Diluent | 1.5 mL × 1 bottle |
| HRP-Conjugate Reagent | 6 mL × 1 bottle |
| Sample Diluent | 6 mL × 1 bottle |
| Chromogen Solution A (TMB A) | 6 mL × 1 bottle |
| Chromogen Solution B (TMB B) | 6 mL × 1 bottle |
| Stop Solution | 6 mL × 1 bottle |
| Wash Buffer (30× Concentrate) | 20 mL × 1 bottle |
| Closure Plate Membrane | 2 pieces |
| User Manual | 1 |
| Sealed Storage Bags | 1 |
Sample Types
- ✅ Rat serum
- ✅ Plasma (EDTA, citrate)
- ✅ Urine
- ✅ Cell culture supernatants or lysates
- ✅ Tissue homogenates
Assay Performance
| Attribute | Value |
|---|---|
| Assay Range | 0.1 – 3.8 ng/mL |
| Sensitivity | 0.01 ng/mL |
| Intra-assay CV | <10% |
| Inter-assay CV | <12% |
| Assay Time | ~2.5 hours |
| Reading | Absorbance at 450 nm |
Applications
🧬 Endocrinology Research – Monitor testosterone levels in hormonal studies
🧪 Reproductive Toxicology – Evaluate testicular function and hormone disruption
🧫 Preclinical Pharmacology – Assess drug effects on hormone levels in vivo
🧠 Behavioral Neuroscience – Study testosterone influence on animal behavior
🔬 Veterinary Research – Investigate hormonal biomarkers in animal models
The Rat Testosterone ELISA Kit (SL0668Ra) provides a sensitive, accurate, and easy-to-use platform for the quantification of testosterone in a wide range of biological fluids. Designed for research use only, this kit is ideal for scientists and labs conducting hormonal, toxicological, or reproductive studies in rat models.
No resources are currently available for this product.
Specifications
| Pack Size | For 48 Tests, For 96 Tests |