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The pRL Renilla Luciferase Control Reporter Vectors are designed for internal control normalization in dual-reporter luciferase assays. They contain wild-type Renilla luciferase (Rluc) cloned from Renilla reniformis (sea pansy) and are intended for co-transfection with firefly luciferase reporter vectors in mammalian cells.
Using Renilla luciferase as an internal control enables accurate normalization of experimental data, correcting for variations in transfection efficiency, sample preparation, and cell health.
Available Vector Variants
| Variant | Promoter | Description | Catalog Number |
|---|---|---|---|
| pRL-null | None | No promoter; allows insertion of custom regulatory elements or use as a negative control vector. | E2271 |
| pRL-CMV | CMV immediate early promoter | Strong expression; ideal for use with low-expression experimental vectors. | E2261 |
| pRL-SV40 | SV40 early promoter/enhancer | Moderate expression for balanced control in most assay setups. | E2231 |
| pRL-TK | HSV thymidine kinase promoter | Weak expression; minimizes interference in co-reporter assays. | E2241 |
Key Features
- Versatile Co-Reporter Tool – Works with firefly luciferase vectors for internal control in mammalian cells.
- Multiple Promoter Strengths – Match expression to experimental requirements.
- Dual-Luciferase Compatibility – Designed for use in firefly/Renilla assays.
- Easy Bacterial Propagation – E. coli origin of replication with β-lactamase gene for ampicillin resistance.
- Restriction Enzyme Flexibility – Prepared in dam⁻/dcm⁻ E. coli K strain for digestion with methylation-sensitive enzymes.
- High-Quality Plasmid DNA – Supplied at 20 μg per vector.
Applications
- Internal control for promoter and enhancer activity assays.
- Normalization in siRNA, miRNA, and transcription factor screening.
- Reporter system for drug discovery and pathway analysis.
- Assay development for high-throughput screening.
Technical Specifications
| Parameter | Details |
|---|---|
| Reporter Gene | Wild-type Renilla luciferase (Rluc) |
| Source | Renilla reniformis (sea pansy) |
| Termination Signal | SV40 late poly(A) |
| Cloning Feature | MCS in pRL-null for custom promoter insertion |
| Vector Backbone | E. coli origin of replication, β-lactamase (ampicillin resistance) |
| Format | 20 μg purified plasmid DNA |
| Bacterial Host | dam⁻/dcm⁻ E. coli K strain |
| Storage | –20°C |
The pRL Renilla Luciferase Control Reporter Vectors provide flexibility, reliability, and precision in luciferase reporter assays. Whether you need high expression for weak experimental constructs or minimal expression to avoid background interference, there’s a pRL vector variant to match your research needs.
pRL Renilla Luciferase Reporter Vectors TB550.pdf
The pRL Renilla Luciferase Control Reporter Vectors are designed for internal control normalization in dual-reporter luciferase assays. They contain wild-type Renilla luciferase (Rluc) cloned from Renilla reniformis (sea pansy) and are intended for co-transfection with firefly luciferase reporter vectors in mammalian cells.
Using Renilla luciferase as an internal control enables accurate normalization of experimental data, correcting for variations in transfection efficiency, sample preparation, and cell health.
Available Vector Variants
| Variant | Promoter | Description | Catalog Number |
|---|---|---|---|
| pRL-null | None | No promoter; allows insertion of custom regulatory elements or use as a negative control vector. | E2271 |
| pRL-CMV | CMV immediate early promoter | Strong expression; ideal for use with low-expression experimental vectors. | E2261 |
| pRL-SV40 | SV40 early promoter/enhancer | Moderate expression for balanced control in most assay setups. | E2231 |
| pRL-TK | HSV thymidine kinase promoter | Weak expression; minimizes interference in co-reporter assays. | E2241 |
Key Features
- Versatile Co-Reporter Tool – Works with firefly luciferase vectors for internal control in mammalian cells.
- Multiple Promoter Strengths – Match expression to experimental requirements.
- Dual-Luciferase Compatibility – Designed for use in firefly/Renilla assays.
- Easy Bacterial Propagation – E. coli origin of replication with β-lactamase gene for ampicillin resistance.
- Restriction Enzyme Flexibility – Prepared in dam⁻/dcm⁻ E. coli K strain for digestion with methylation-sensitive enzymes.
- High-Quality Plasmid DNA – Supplied at 20 μg per vector.
Applications
- Internal control for promoter and enhancer activity assays.
- Normalization in siRNA, miRNA, and transcription factor screening.
- Reporter system for drug discovery and pathway analysis.
- Assay development for high-throughput screening.
Technical Specifications
| Parameter | Details |
|---|---|
| Reporter Gene | Wild-type Renilla luciferase (Rluc) |
| Source | Renilla reniformis (sea pansy) |
| Termination Signal | SV40 late poly(A) |
| Cloning Feature | MCS in pRL-null for custom promoter insertion |
| Vector Backbone | E. coli origin of replication, β-lactamase (ampicillin resistance) |
| Format | 20 μg purified plasmid DNA |
| Bacterial Host | dam⁻/dcm⁻ E. coli K strain |
| Storage | –20°C |
The pRL Renilla Luciferase Control Reporter Vectors provide flexibility, reliability, and precision in luciferase reporter assays. Whether you need high expression for weak experimental constructs or minimal expression to avoid background interference, there’s a pRL vector variant to match your research needs.
pRL Renilla Luciferase Reporter Vectors TB550.pdf
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Specifications
| Vector | pRL-null vector, pRL-CMV vector, pRL-SV40 vector, pRL-TK vector |