Promega dNTP Mix 10mM

The Promega dNTP Mix is a high-purity, ready-to-use nucleotide solution designed for PCR, RT-PCR, qPCR, DNA sequencing, and other enzymatic reactions. This solution simplifies experimental workflows by reducing pipetting steps and minimizing contamination risks.

It consists of four highly purified (>99%) deoxynucleotide triphosphates (dATP, dCTP, dGTP, and dTTP), each at 10mM concentration in water (pH 7.5), resulting in a total dNTP concentration of 40mM.

🔹 Supplied in two convenient sizes:

• U1511 – 200 µL
• U1515 – 1,000 µL (1 mL)

📌 Usage: Adding 1 µL of dNTP Mix to a 50 µL PCR reaction results in a final concentration of 200 µM per dNTP.

Key Features & Benefits

1. High Purity for Accurate DNA Amplification

✔ >99% Triphosphate Purity – Ensures efficient and accurate polymerase activity, reducing unwanted side reactions.

✔ RNase/DNase-Free – Prevents degradation of DNA and RNA templates, ensuring high-fidelity amplification.

✔ Optimized pH (7.5) – Maintains enzyme stability and performance during reactions.

2. Ready-to-Use, Pre-Mixed Solution

✔ Convenient 10mM Concentration of Each dNTP – Eliminates the need for individual nucleotide preparation.

✔ Reduces Pipetting Errors – Ensures accurate and reproducible reaction setups.

✔ Minimizes Contamination Risks – Reduces handling steps and cross-contamination possibilities.

3. Ideal for a Wide Range of Applications

✔ Compatible with Taq, Pfu, and High-Fidelity Polymerases – Works with various thermostable and proofreading enzymes.

✔ Supports High-Yield DNA Synthesis – Essential for cloning, sequencing, and amplification of long templates.

✔ qPCR and RT-PCR Ready – Ensures consistent nucleotide incorporation in real-time PCR workflows.

Technical Specifications

Applications

1. Polymerase Chain Reaction (PCR) & Quantitative PCR (qPCR)

🧬 Standard PCR & qPCR – Provides reliable and consistent amplification.

🧬 High-Fidelity & Long PCR – Works with proofreading polymerases for accurate amplicons.

🧬 Multiplex PCR – Supports amplification of multiple targets in a single reaction.

2. Reverse Transcription PCR (RT-PCR) & cDNA Synthesis

🦠 RNA-Based Applications – Essential for cDNA synthesis and gene expression analysis.

🦠 Compatible with Reverse Transcriptases – Works with M-MLV, AMV, and other reverse transcriptases.

3. DNA Sequencing & Cloning

🔬 Sanger Sequencing – Provides high-quality DNA polymerase activity for sequencing workflows.

🔬 Next-Generation Sequencing (NGS) Library Prep – Ensures accurate nucleotide incorporation for DNA fragmentation and amplification steps.

🔬 Ligation & Cloning – Supports accurate DNA synthesis for restriction digest-based cloning.

4. Genotyping & SNP Detection

🧬 SNP Genotyping – Provides high-purity nucleotides for mutation detection assays.

🧬 Allelic Discrimination Studies – Supports accurate base incorporation for sequence-specific applications.

Usage Guidelines

✔ Thaw at room temperature before use.

✔ Vortex gently before pipetting to ensure uniformity.

✔ Use 1 µL per 50 µL reaction to achieve a final 200 µM dNTP concentration.

✔ Store at -20°C to maintain stability.

✔ Avoid repeated freeze-thaw cycles.

Ordering Information

📦 Shipping Conditions: Dry Ice

🧊 Storage: -20°C

Why Choose Promega dNTP Mix?

✅ High Purity & Quality – Ensures accurate and efficient DNA synthesis.

✅ Pre-Mixed for Convenience – Saves time and reduces pipetting errors.

✅ Compatible with Multiple Applications – Works for PCR, RT-PCR, qPCR, sequencing, and cloning.

✅ Reliable & Trusted Brand – Manufactured under strict quality control standards.

The Promega dNTP Mix is a high-quality, ready-to-use nucleotide solution designed for accurate and reproducible DNA amplification in PCR, sequencing, cloning, and genotyping applications. Its pre-mixed format eliminates preparation steps, reducing contamination risks and improving workflow efficiency.

🚀 Ideal for: Molecular biologists, geneticists, microbiologists, forensic scientists, and clinical researchers conducting high-quality PCR and DNA amplification assays.