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Specifications:
| Application | Protein Biology | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Biochemical Reagent | Forms | Powder |
| Product Brand | MedChem Express | ||
| Product Grade | Analytical grade | Formula | C36H56O8 |
Phorbol 12-myristate 13-acetate (PMA), a phorbol ester, is a dual SphK and protein kinase C (PKC) activator. Phorbol 12-myristate 13-acetate is a NF-κB activator. Phorbol 12-myristate 13-acetate induces differentiation in THP-1 cells.
| Description | Phorbol 12-myristate 13-acetate (PMA), a phorbol ester, is a dual SphK and protein kinase C (PKC) activator[1][2]. Phorbol 12-myristate 13-acetate is a NF-κB activator. Phorbol 12-myristate 13-acetate induces differentiation in THP-1 cells[3][7]. | ||
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| IC50 & Target[1][7] |
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| In Vitro | PMA (200 ng/mL; 1-5 days) induce THP-1 cells to differentiate into macrophage-like cells (THP-1 macrophages), characterized by changes in morphology (adherent macrophage-like phenotype), and increases cell surface expression of CD11 and CD14[3][5]. PMA (20 ng/mL, 36 h) inhibits endothelial cell migration through activating the PKC-δ/Syk/NF-κB-mediated up-regulation of Thy-1[8]. MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Phorbol 12-myristate 13-acetate Related Antibodies
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| In Vivo | Phorbol 12-myristate 13-acetate can be used in animal modeling to construct eczema-like models. Phorbol 12-myristate 13-acetate (PMA) is a PKC agonist, which reverses the damage induced by 5-hydroxydecanoic acid (5-HD). Thus, activation of the mitoKATP protected mitochondrial function in SOD and MDA via the PKC pathway[4].1. Induction of oedema at ear[8]BackgroundPMA induces a pronounced inflammatory response mediated by protein kinase C (PKC), specifically activating PLA2 to trigger inflammation.Specific Mmodeling MethodsMice: Swiss mouse • Female • 25-30 g Administration: Topically applied in one ear • 100 μg/mL in 20 μL (2 μg/ear) vehicle • single dose Modeling IndicatorsAppearance monitoring: The thickness difference between the left and right ears increases significantly. Indicator changes: Increased vascular permeability.Opposite Product(s): Hydroxyachillin; Indomethacin (HY-14397)2. Induction of oedema at feet[9]BackgroundPMA induces a pronounced inflammatory response mediated by protein kinase C (PKC), specifically activating PLA2 to trigger inflammation.Specific Mmodeling MethodsRats: Wistar • male • adult with weight of 200-220 g Mice: Swiss albino • male • 25-30 g Administration: Topically applied in one ear • 2.5 μg in 20 μL vehicle • single doseNoteAdministration should be conducted 4 h before mouse were killed. Modeling Indicators Appearance monitoring: The quality difference between the left and right ears increases significantly. Indicator changes: Stimulate macrophages to produce superoxide anions. |
- Pack Size: 10mg 5mg 25mg 1mg 10 mM x 1 mL in DMSO