Percoll™ density gradient media

Cytiva Percoll™ is a colloidal silica-based density gradient medium coated with polyvinylpyrrolidone (PVP), designed for the isolation and purification of cells, organelles, viruses, and subcellular particles.

Its unique composition allows formation of self-generated or pre-formed gradients in balanced salt solutions, making it one of the most versatile and widely used reagents for density gradient centrifugation in cell biology and biochemistry research.

Percoll™ is isotonic, non-toxic, and biologically inert, enabling gentle and efficient separations without altering cell morphology or function.

Key Features

• Versatile Density Gradient Medium: Suitable for separation of cells, organelles, and macromolecular complexes.
• Silica-PVP Composition: Colloidal silica particles (15–30 nm) coated with polyvinylpyrrolidone for biological compatibility.
• Adjustable Gradients: Can form continuous or step gradients by centrifugation or gradient mixers.
• Physiological Compatibility: Used with balanced salt solutions, 0.25 M sucrose, or physiological saline.
• Biologically Inert and Isotonic: Maintains osmotic balance and cell integrity during centrifugation.
• High Recovery and Viability: Enables efficient cell separation with minimal mechanical or osmotic stress.
• Colorless to Slightly Yellow: Clear colloidal suspension for easy gradient visualization.
• Certificate of Analysis (CoA) available upon request.

Specifications

Applications

• Cell Separation: Isolation of lymphocytes, macrophages, neutrophils, and other blood or tissue cells.
• Organelle Fractionation: Separation of mitochondria, lysosomes, nuclei, and peroxisomes.
• Virology: Purification of viruses and subviral particles.
• Subcellular Analysis: Fractionation of cellular components for proteomic or biochemical assays.
• Density Gradient Studies: Formation of continuous or discontinuous gradients for macromolecular separations.

Advantages

• Gentle on delicate cell types
• Reproducible and consistent density gradients
• Compatible with a wide range of centrifuge rotors
• Suitable for both analytical and preparative separations
• Long shelf life and batch-to-batch consistency

The Cytiva Percoll™ medium is a silica-based, PVP-coated density gradient reagent ideal for biological particle separation via centrifugation.

Offering exceptional reproducibility, compatibility, and efficiency, it remains a gold standard for cell, organelle, and virus purification in research laboratories worldwide.

Trusted for its biocompatibility, isotonicity, and performance, Percoll™ continues to be a foundational reagent in cell biology, virology, and molecular biochemistry research.