Invitrogen™ alpha Amylase Polyclonal Antibody, HRP, 1mg

The Invitrogen™ Alpha Amylase Polyclonal Antibody, HRP is a highly specific rabbit-derived IgG antibody conjugated to horseradish peroxidase (HRP). This antibody is designed for detecting alpha-amylase, particularly from Bacillus amyloliquefaciens, in various research applications such as Western blot, ELISA, and immunoprecipitation.

Key Features:

1. Specificity:
   • Recognizes alpha-amylase, an enzyme responsible for hydrolyzing 1,4-alpha-glucoside bonds in oligosaccharides and polysaccharides.
2. Applications:
   • Validated for use in:
     • Western Blot (WB): 1:500–1:5,000.
     • ELISA: 1:5,000–1:20,000.
     • Immunoprecipitation (IP): 1:100.
3. HRP Conjugation:
   • Conjugated with horseradish peroxidase (HRP), enabling sensitive and specific detection in enzyme-based assays.
4. High Purity:
   • IgG fraction purified to ensure minimal background noise and high specificity.
5. Broad Range of Reactivity:
   • Specifically reacts with bacterial alpha-amylase, particularly from Bacillus amyloliquefaciens.

Product Specifications:

Applications:

1. Western Blot (WB):
   • Detects alpha-amylase in bacterial protein samples with high sensitivity.
   • Dilution: 1:500–1:5,000.
2. ELISA:
   • Quantitative or qualitative detection of alpha-amylase in complex samples.
   • Dilution: 1:5,000–1:20,000.
3. Immunoprecipitation (IP):
   • Precipitates alpha-amylase from bacterial lysates for downstream analyses.
   • Dilution: 1:100.

Use Cases:

1. Protein Expression Studies:
   • Investigates alpha-amylase production and activity in Bacillus amyloliquefaciens or other bacterial species.
2. Enzymatic Research:
   • Supports studies on carbohydrate metabolism and enzymatic hydrolysis of starch and glycogen.
3. Biotechnology Applications:
   • Evaluates alpha-amylase activity in industrial strains used for biofuel, brewing, or food production.
4. Comparative Analyses:
   • Compares alpha-amylase levels across different bacterial strains or growth conditions.
5. Purification and Activity Validation:
   • Confirms purity and functionality of alpha-amylase in recombinant protein production.

Advantages:

1. High Specificity and Sensitivity:
   • Accurate detection of bacterial alpha-amylase with minimal background interference.
2. Versatile Conjugation:
   • HRP conjugation allows direct detection in enzyme-linked assays and Western blotting.
3. Wide Applicability:
   • Suitable for multiple techniques, including WB, ELISA, and IP.
4. Stability:
   • Long shelf life with proper storage, ensuring consistent performance.

Storage and Handling:

• Short-Term Storage: Store at 4°C for immediate use.
• Long-Term Storage: Store at -20°C to preserve antibody integrity; avoid repeated freeze/thaw cycles.
• Shipping Conditions: Shipped on wet ice to maintain product quality during transit.

Product Details:

The Invitrogen™ Alpha Amylase Polyclonal Antibody, HRP is an essential tool for researchers investigating alpha-amylase in bacterial systems. Its high specificity, broad applications, and HRP conjugation make it ideal for enzymatic, structural, and functional studies.