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The Invitrogen™ Protein G–HRP Conjugate is a highly versatile immunoreagent designed for the sensitive and specific detection of immunoglobulins through interaction with the Fc region. Conjugated with horseradish peroxidase (HRP), Protein G enables enzymatic detection via chromogenic, chemiluminescent, or fluorescent substrates in immunoassays such as ELISA, Western blotting, and immunohistochemistry.
Protein G, derived from Streptococcus species, exhibits strong and selective binding to the Fc region of a wide range of mammalian IgG subclasses, with superior affinity compared to Protein A—particularly for mouse and human IgG.
Key Features
- Broad Species Reactivity: Binds to IgG from human, mouse, rabbit, goat, sheep, and other mammalian species.
- High Sensitivity: HRP conjugate provides strong signal output in enzyme-linked detection formats.
- Improved Selectivity: Greater affinity for mouse IgG1 and human IgG3 compared to Protein A.
- Versatile Applications: Ideal for ELISA, immunoblotting, dot blots, immunohistochemistry, and other Fc-based detection methods.
Specifications
| Attribute | Details |
|---|---|
| Form | Lyophilized powder |
| Conjugate | Horseradish peroxidase (HRP) |
| Reactivity | Binds Fc region of IgG from many species |
| Quantity | 1 mg |
| Storage | –5 to –30 °C (freeze upon receipt) |
| Shipping Condition | Room temperature |
| Unit Size | Each |
Recommended Applications
- Western Blotting – Detection of primary IgG antibodies via chemiluminescent substrates
- ELISA – As a secondary reagent in indirect or sandwich ELISA formats
- Immunohistochemistry (IHC) – Enzyme-based tissue detection of IgG-bound targets
- Dot and Slot Blotting – For membrane-based detection of bound antibodies
Storage & Reconstitution
Upon receipt, store lyophilized product at –5 °C to –30 °C. Reconstitute using sterile water or an appropriate buffer as recommended in your specific assay protocol. Aliquot and avoid repeated freeze-thaw cycles for maximum stability.
The Invitrogen™ Protein G–HRP Conjugate provides high-performance antibody detection across diverse platforms. Its enhanced affinity for IgG subclasses and stable enzyme conjugation make it an essential tool for researchers in immunology, diagnostics development, and antibody-based assay systems.
User Guide
The Invitrogen™ Protein G–HRP Conjugate is a highly versatile immunoreagent designed for the sensitive and specific detection of immunoglobulins through interaction with the Fc region. Conjugated with horseradish peroxidase (HRP), Protein G enables enzymatic detection via chromogenic, chemiluminescent, or fluorescent substrates in immunoassays such as ELISA, Western blotting, and immunohistochemistry.
Protein G, derived from Streptococcus species, exhibits strong and selective binding to the Fc region of a wide range of mammalian IgG subclasses, with superior affinity compared to Protein A—particularly for mouse and human IgG.
Key Features
- Broad Species Reactivity: Binds to IgG from human, mouse, rabbit, goat, sheep, and other mammalian species.
- High Sensitivity: HRP conjugate provides strong signal output in enzyme-linked detection formats.
- Improved Selectivity: Greater affinity for mouse IgG1 and human IgG3 compared to Protein A.
- Versatile Applications: Ideal for ELISA, immunoblotting, dot blots, immunohistochemistry, and other Fc-based detection methods.
Specifications
| Attribute | Details |
|---|---|
| Form | Lyophilized powder |
| Conjugate | Horseradish peroxidase (HRP) |
| Reactivity | Binds Fc region of IgG from many species |
| Quantity | 1 mg |
| Storage | –5 to –30 °C (freeze upon receipt) |
| Shipping Condition | Room temperature |
| Unit Size | Each |
Recommended Applications
- Western Blotting – Detection of primary IgG antibodies via chemiluminescent substrates
- ELISA – As a secondary reagent in indirect or sandwich ELISA formats
- Immunohistochemistry (IHC) – Enzyme-based tissue detection of IgG-bound targets
- Dot and Slot Blotting – For membrane-based detection of bound antibodies
Storage & Reconstitution
Upon receipt, store lyophilized product at –5 °C to –30 °C. Reconstitute using sterile water or an appropriate buffer as recommended in your specific assay protocol. Aliquot and avoid repeated freeze-thaw cycles for maximum stability.
The Invitrogen™ Protein G–HRP Conjugate provides high-performance antibody detection across diverse platforms. Its enhanced affinity for IgG subclasses and stable enzyme conjugation make it an essential tool for researchers in immunology, diagnostics development, and antibody-based assay systems.