Thermo Scientific™ Applied Biosystems™ MicroAmp™ 8-Cap Strip, Dome shaped, clear

Applied Biosystems™ MicroAmp™ 8-Cap Strips are designed for secure sealing of MicroAmp™ PCR tubes, strip tubes, and 96-well plates. These high-quality polypropylene domed caps provide a tight seal to minimize sample evaporation and contamination, ensuring reliable PCR and qPCR performance.

They are compatible with 0.2 mL individual tubes, strip tubes (0.1 or 0.2 mL), and 48- or 96-well plates, making them ideal for high-throughput PCR, endpoint PCR, and DNA amplification workflows.

For real-time PCR applications, the MicroAmp™ Optical 8-Cap Strips (Cat. No. 4323032) with a flat-top profile are recommended to ensure optical clarity and fluorescence signal detection.

Key Features & Benefits

1. Tight-Sealing Caps to Prevent Evaporation & Contamination
   • Maintains sample integrity during PCR thermal cycling.
   • Provides a reliable seal to minimize evaporation and cross-contamination.
2. Domed Profile for Enhanced Performance in Endpoint PCR
   • Suitable for conventional PCR assays and gel electrophoresis analysis.
   • Improves thermal sealing while preventing condensation buildup.
3. Compatible with Various MicroAmp™ Reaction Tubes & Plates
   • Fits 0.2 mL individual PCR tubes, 8-strip tubes, and 96-well plates.
   • Ensures seamless integration with Applied Biosystems™ thermal cyclers.
4. Easy Application & Removal
   • Designed to be used with the MicroAmp™ Cap Installing Tool for quick sealing.
   • Easily removed using the built-in tabs at the end of each strip.
5. Certified DNA/RNase-Free for Contamination-Free PCR
   • Prevents sample degradation and ensures reliable molecular biology results.
   • Suitable for sensitive PCR applications, including genotyping, sequencing, and forensics.

Product Specifications

Use Cases & Applications

1. Polymerase Chain Reaction (PCR) & Quantitative PCR (qPCR)

• Ensures Secure Sealing During Thermal Cycling
  • Prevents sample evaporation and cross-contamination.
  • Compatible with high-throughput PCR workflows.
• Supports Endpoint PCR & Gel Electrophoresis Analysis
  • Domed cap design allows efficient condensation control.
  • Ideal for genotyping, sequencing, and DNA amplification assays.

2. Real-Time PCR & Fluorescence-Based Detection

• Compatible with MicroAmp™ PCR Strip Tubes & 96-Well Plates
  • Works well with Applied Biosystems™ real-time PCR systems.
  • Provides consistent fluorescence signal detection in qPCR assays.
• Alternative Option for qPCR Optical Caps
  • For qPCR fluorescence-based assays, use MicroAmp™ Optical 8-Cap Strips (4323032) for optimal signal clarity.

3. Molecular Biology & DNA/RNA Research

• Securely Seals Nucleic Acid Samples for Amplification
  • Ideal for RT-PCR, cDNA synthesis, and pathogen detection assays.
  • Prevents sample degradation and contamination during sensitive workflows.
• Supports Microbial & Viral DNA/RNA Testing
  • Used in forensic, diagnostic, and clinical molecular assays.
  • Ensures high reproducibility and assay reliability.

4. Genomics & Biotechnological Research

• Facilitates High-Throughput Genotyping & SNP Analysis
  • Ensures efficient processing of multiple samples in parallel.
  • Provides secure sample handling in automated workflows.
• Enhances Sample Processing in Next-Generation Sequencing (NGS) Workflows
  • Reliable for pre-PCR sample amplification and DNA library preparation.
  • Facilitates targeted sequencing workflows with high reproducibility.

Usage & Handling Instructions

Cap Installation & Sample Preparation

1. Place PCR Tubes or Plates in the Thermal Cycler Block
   • Ensure that all reaction tubes are properly positioned for uniform heating.
2. Seal with MicroAmp™ 8-Cap Strips
   • Use the MicroAmp™ Cap Installing Tool to apply even pressure and form a secure seal.
   • Ensure caps are tightly closed to prevent evaporation during PCR cycling.
3. Run the Thermal Cycling Program
   • Use the recommended PCR/qPCR conditions for accurate and reproducible amplification.
4. Post-PCR Sample Handling & Analysis
   • Remove caps using the built-in tabs for easy opening.
   • Proceed with gel electrophoresis, qPCR analysis, or sequencing workflows.

Storage & Handling Instructions

• Storage Conditions:
  • Store at room temperature in a dry, contamination-free environment.
  • Keep caps sealed in the original packaging until use.
• Handling Precautions:
  • Use gloves and aseptic techniques to prevent contamination.
  • Ensure proper sealing of caps to prevent sample loss.
• Disposal Guidelines:
  • Dispose of used cap strips as per biosafety and laboratory waste regulations.
  • If used with biohazardous materials, autoclave before disposal.

Why Choose Applied Biosystems™ MicroAmp™ 8-Cap Strips?

✅ Designed for PCR, qPCR, and molecular biology workflows

✅ Domed profile minimizes evaporation & prevents contamination

✅ Certified DNA/RNase-free for contamination-free amplification

✅ Compatible with 0.2 mL PCR tubes, strip tubes, and 96-well plates

✅ Easy-to-apply and remove, with optional cap installation tool

Applied Biosystems™ MicroAmp™ 8-Cap Strips are a reliable choice for secure and precise sample sealing, ensuring consistent amplification and contamination-free PCR and qPCR workflows.