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Specifications:
| Application | Analytical Chemistry, Colorimetric Assay | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Laboratory Chemical | Forms | Powder |
| Product Brand | MedChem Express | ||
| Product Grade | Analytical grade | Formula | C₁₂H₁₇N₃O₃ |
The MCE L-Leucyl-4-nitroanilide (Leu-pNA) is a high-purity chromogenic substrate specifically designed for leucine aminopeptidase (LAP) activity assays. With an exceptional purity of ≥99.95%, this compound delivers highly sensitive and reproducible results in enzyme kinetics, inhibitor screening, and biochemical research applications.
Upon enzymatic cleavage, L-Leucyl-4-nitroanilide releases p-nitroaniline (pNA), producing a yellow chromophore that can be quantitatively measured using spectrophotometry. This makes it a reliable and convenient reagent for colorimetric enzyme assays, enabling accurate monitoring of enzymatic activity in real time.
Manufactured by MedChemExpress (MCE), this reagent is optimized for research applications and is suitable for use in life sciences, pharmaceutical research, and enzymology studies.
Key Features
- Ultra-high purity (≥99.95%) for enhanced analytical accuracy
- Specifically designed for leucine aminopeptidase (LAP) assays
- Chromogenic substrate releasing p-nitroaniline (pNA) upon cleavage
- Enables colorimetric detection via spectrophotometry
- Compatible with enzyme kinetics and inhibitor screening workflows
- Flexible packaging options from 100 mg to 25 g
- Reliable product from MedChemExpress (MCE)
Applications
- Leucine aminopeptidase (LAP) activity assays
- Protease and peptidase enzyme studies
- Enzyme kinetics and catalytic activity analysis
- Drug discovery and inhibitor screening
- Biochemical and molecular biology research
- Substrate specificity investigations
Technical Specifications
| Parameter | Specification |
|---|---|
| Product Name | L-Leucyl-4-nitroanilide |
| Synonyms | H-Leu-pNA; L-Leucine-p-nitroanilide |
| CAS Number | 4178-93-2 |
| Molecular Formula | C₁₂H₁₇N₃O₃ |
| Molecular Weight | 251.28 g/mol |
| Purity | ≥99.95% |
| Appearance | Solid |
| Color | Light yellow to yellow |
| Solubility | DMSO (up to 100 mg/mL with sonication) |
| Storage (solid) | Room temperature (up to 3 years) |
| Storage (solution) | -20°C (1 year), -80°C (2 years) |
| Shipping | Room temperature |
| Pack Sizes | 100 mg, 250 mg, 500 mg, 1 g, 5 g, 10 g, 25 g |
Advantages
The MCE L-Leucyl-4-nitroanilide offers exceptional sensitivity and stability, making it ideal for detecting even low levels of enzyme activity. Its high purity ensures minimal background interference, while the chromogenic pNA release enables simple and rapid absorbance-based measurements without the need for complex detection systems.
Additionally, its flexible storage conditions and high solubility in DMSO allow easy preparation of stock solutions for a wide range of experimental setups, improving workflow efficiency and reproducibility.
The MCE L-Leucyl-4-nitroanilide (≥99.95%) is a premium-grade chromogenic substrate tailored for leucine aminopeptidase assays and enzyme research. Combining ultra-high purity, reliable performance, and ease of use, it is an essential reagent for laboratories focused on enzymology, drug discovery, and biochemical analysis.
- Pack Size: 10 g 100 mg 1 g 500 mg 250 mg 25 g 5 g