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Thermo Scientific™ Oxoid™ Microbact™ Oxidase Detection Strips
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Specifications:
| Application | Microbiology |
| Storage Temperature | 2-8°C |
| Product Type | Biochemical Reagent |
| Product Brand | Thermo Fisher Scientific™, Oxoid |
| Product Grade | Microbiology grade |
The Thermo Scientific™ Oxoid™ Microbact™ Oxidase Detection Strips (MB0266) are a convenient and reliable tool for detecting oxidase activity in bacterial isolates. These strips are impregnated with tetramethyl-p-phenylenediamine dihydrochloride, which reacts with cytochrome c oxidase enzymes to produce a distinctive color change, enabling the identification of oxidase-positive organisms.
Key Features
- Rapid Detection:
- Provides results within 30 seconds to 1 minute, streamlining workflows in clinical and research laboratories.
- User-Friendly Format:
- Supplied as convenient strips, eliminating the need for complex reagent preparation.
- High Sensitivity:
- Detects cytochrome c oxidase activity in oxidase-positive organisms, ensuring reliable identification.
- Clear Visual Results:
- Positive reaction: Purple/blue color change.
- Negative reaction: No color change.
- Broad Applicability:
- Suitable for testing a wide range of Gram-negative bacteria, including Pseudomonas, Neisseria, and Moraxella spp.
Applications
1. Clinical Microbiology
- Differentiation of oxidase-positive pathogens, such as Pseudomonas aeruginosa, from oxidase-negative Enterobacteriaceae.
2. Food Microbiology
- Identification of spoilage bacteria and pathogens in food products.
3. Environmental Microbiology
- Testing environmental isolates for oxidase activity.
4. Research Microbiology
- Studies involving metabolic activity and respiratory enzyme systems in bacteria.
Specifications
| Attribute | Details |
|---|---|
| Product Type | Oxidase Detection Strips |
| Reagent | Tetramethyl-p-phenylenediamine dihydrochloride |
| Applications | Clinical, food, environmental, and research microbiology |
| Form | Pre-impregnated strips |
| Reaction Time | 30 seconds to 1 minute |
| Storage Conditions | 2–8°C |
| Unit Size | Pack of 50 strips |
Procedure
- Sample Application:
- Remove a strip from the container. Using a sterile applicator, smear a colony from a fresh bacterial culture onto the strip.
- Observe Reaction:
- Observe the strip for color change within 30 seconds to 1 minute.
- Interpret Results:
- Positive Reaction: Purple/blue color indicates oxidase-positive bacteria.
- Negative Reaction: No color change indicates oxidase-negative bacteria.
Advantages
- Ease of Use: Pre-impregnated strips simplify the oxidase testing process.
- Quick Results: Provides a rapid and reliable method for bacterial differentiation.
- No Reagent Preparation: Ready-to-use format eliminates reagent preparation steps.
- Portable: Compact design makes it suitable for field and laboratory use.
- Broad Compatibility: Effective across various bacterial isolates, enhancing versatility.
Quality Control
| Control | Expected Results |
|---|---|
| Positive Control | Pseudomonas aeruginosa – Purple/blue reaction |
| Negative Control | Escherichia coli – No color change |
Storage and Stability
- Storage: Store at 2–8°C in the original, sealed container to prevent exposure to moisture and light.
- Shelf Life: Refer to product label for expiration date.
Packaging
- Product: Microbact™ Oxidase Detection Strips
- Catalog Number: MB0266
- Quantity: 50 strips per pack
Conclusion
The Thermo Scientific™ Oxoid™ Microbact™ Oxidase Detection Strips (MB0266) offer a rapid, reliable, and easy-to-use solution for detecting oxidase activity in bacterial isolates. Their convenience, sensitivity, and compatibility with diverse applications make them indispensable for microbiology laboratories focused on clinical, food, environmental, and research testing.