Thermo Scientific™ Oxoid™ Agar Bacteriological

Thermo Scientific™ Oxoid™ Agar Bacteriological is a high-quality processed agar with superior gel strength and low mineral content, specifically designed for microbiological applications requiring consistent and reliable performance.

Key Features:

1. High Gel Strength:
   • Provides a 1.5% w/v working gel strength, ensuring firm and uniform agar formulations.
2. Low Calcium (Ca) and Magnesium (Mg) Content:
   • Ideal for applications where mineral and metal content must be controlled, such as antimicrobial susceptibility testing.
   • Prevents interference in Minimum Inhibitory Concentration (MIC) studies by maintaining consistent mineral values across broth and agar formulations.
3. Wide Compatibility:
   • Suitable for use with all culture media, enabling consistent microbial growth and assay performance.
4. Optimal for Antimicrobial Diffusion Studies:
   • Low mineral and metal content ensures free diffusion of antimicrobial agents, making it an excellent choice for disc diffusion susceptibility tests.
5. Enhanced Accuracy in MIC Studies:
   • Eliminates variability caused by differing mineral/metal contents, ensuring reliable and reproducible results in antimicrobial MIC studies.

Applications:

1. Antimicrobial Susceptibility Testing (AST):
   • Perfect for disc diffusion tests, ensuring accurate zone of inhibition measurements.
2. Minimum Inhibitory Concentration (MIC) Studies:
   • Maintains consistent mineral content between broth and agar, reducing variability.
3. Microbial Culture:
   • Compatible with a wide range of culture media formulations, supporting diverse microbial growth requirements.
4. General Microbiology:
   • Suitable for routine microbiological assays and specialized research applications.

Specifications:

Preparation:

1. Suspend 15 g of agar in 1 liter of distilled water.
2. Heat to boiling while stirring to ensure complete dissolution.
3. Sterilize by autoclaving at 121°C for 15 minutes.
4. Cool to 45-50°C before pouring into Petri dishes or other containers.

Advantages:

• Ensures consistent performance in sensitive microbial studies.
• Reduces variability in antimicrobial studies caused by mineral content differences.
• Supports a wide range of laboratory applications, from routine to specialized research.

Storage and Shelf Life:

• Storage: Store the dehydrated agar at 10-30°C in a tightly sealed container to avoid contamination and moisture absorption.
• Shelf Life: Use before the expiry date indicated on the label for optimal performance.

This product is a reliable choice for microbiological laboratories focused on precision, consistency, and reproducibility in their antimicrobial and microbial studies.