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Liofilchem™ MTS™ Daptomycin DAP 0.016-256, MIC Test strips
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Specifications:
| Application | Clinical microbiology |
| Storage Temperature | -20°C |
| Product Type | MIC Strips |
| Product Brand | Liofilchem |
| Product Grade | Microbiology grade |
The Liofilchem™ MTS™ Daptomycin (DAP) 0.016–256 μg/mL MIC Test Strips are specialized tools designed for the precise determination of the Minimum Inhibitory Concentration (MIC) of daptomycin against various microorganisms. These strips are integral in assessing microbial susceptibility, guiding effective antimicrobial therapy, and monitoring resistance patterns.
Product Overview:
- Brand: Liofilchem™
- Product Line: MIC Test Strips (MTS™)
- Antimicrobial Agent: Daptomycin (DAP)
- Concentration Range: 0.016 to 256 μg/mL
- Purpose: Quantitative determination of MIC for daptomycin
Technical Specifications:
| Specification | Details |
|---|---|
| Material | High-quality paper impregnated with a predefined gradient of daptomycin |
| Gradient Range | 0.016 to 256 μg/mL |
| Format | Strips with MIC scale in μg/mL and antimicrobial code |
| Packaging Options | Available in packs of 10, 30, or 100 strips |
| Storage Conditions | Store at 2–8°C in a dry environment. Store at -20°C for optimal stability over extended periods. |
| Shelf Life | Refer to the product's expiration date on the packaging |
Test Procedure:
- Inoculum Preparation:
- Select well-isolated colonies from an overnight culture.
- Suspend the colonies in sterile saline or broth to achieve a turbidity equivalent to a 0.5 McFarland standard.
- Inoculation of Agar Plate:
- Dip a sterile swab into the prepared inoculum suspension.
- Remove excess liquid by pressing the swab against the tube wall.
- Evenly streak the swab over the entire surface of a Mueller-Hinton agar plate to ensure a uniform lawn of growth.
- Application of MTS™ Strip:
- Allow the inoculated plate to dry for approximately 15 minutes at room temperature.
- Using sterile forceps, place the MTS™ Daptomycin strip onto the agar surface with the concentration scale facing upward.
- Ensure complete contact between the strip and the agar surface; do not reposition once applied.
- Incubation:
- Incubate the plate in an inverted position at 35 ± 2°C for 16–20 hours under ambient atmospheric conditions.
- Reading and Interpretation:
- After incubation, observe the plate for an elliptical zone of inhibition around the strip.
- Determine the MIC by identifying the point on the scale where the edge of the inhibition ellipse intersects the strip.
- Interpret the MIC value using established clinical breakpoints from CLSI or EUCAST guidelines.
Quality Control:
To ensure the accuracy and reliability of the test, perform quality control using standard reference strains with known MIC values. For daptomycin, the following quality control strains are recommended:
| Organism | ATCC® Number | Expected MIC Range (μg/mL) |
|---|---|---|
| Staphylococcus aureus | ATCC® 29213 | 0.25–1 |
| Enterococcus faecalis | ATCC® 29212 | 1–4 |
Regular testing of these control strains ensures that the test conditions are optimal and that the MTS™ strips are performing within specified parameters. If the MIC values obtained for the control strains fall outside the expected range, investigate potential issues with the testing procedure, reagents, or equipment.
References:
- Liofilchem® MIC Test Strip Application Guide: https://www.liofilchem.net/pdf/mic/MIC_APPLICATION_GUIDE.pdf
- EUCAST Clinical Breakpoints: https://www.eucast.org/clinical_breakpoints
For detailed procedures, quality control ranges, and interpretative criteria, always refer to the manufacturer's instructions and the latest CLSI and EUCAST guidelines.
- Pack Size: 100/pk 10/pk 30/pk