Liofilchem® Lauryl Tryptose Broth

The Liofilchem® Lauryl Tryptose Broth is a selective medium designed for detecting coliform organisms in water and wastewater, as per the American Public Health Association (APHA) standards. This reliable and easy-to-prepare broth is essential for microbial testing in water quality and environmental monitoring.

Key Features

1. Selective Detection:
   • Specifically formulated for isolating coliforms in water and wastewater.
2. Reliable Indicators:
   • Turbidity and gas production confirm the presence of coliforms.
3. Selective Agent:
   • Sodium lauryl sulphate inhibits non-coliform organisms, ensuring specificity.
4. Convenient Preparation:
   • Dehydrated powder for simple and efficient reconstitution.

Specifications

Formula

Physical Properties

Environmental and Storage

Packaging Options

Applications

1. Water Quality Testing:
   • Reliable detection of coliforms in potable water and wastewater.
2. Environmental Monitoring:
   • Ensures compliance with water safety regulations.
3. Industrial Applications:
   • Testing water used in food, beverage, and pharmaceutical industries.

Usage Guidelines

Preparation

1. Suspend 35.6 g of powder in 1 liter of distilled or deionized water.
2. Heat until completely dissolved.
3. Dispense into final containers with Durham tubes.
4. Autoclave at 121°C for 15 minutes.

Technique

1. Inoculate 1 mL of sample (or serial dilutions) into a tube of Lauryl Tryptose Broth.
2. Invert the tube to expel air from the Durham tube.
3. Incubate at 36 ± 1°C for 24-48 hours.

Results Interpretation

• Positive Presumptive Test: Turbidity and gas production.
• Confirmation: Perform the indole test directly in the tube.

Microbial Performance

Advantages

1. Selective Growth:
   • Sodium lauryl sulphate ensures the inhibition of non-coliform organisms.
2. Ease of Use:
   • Dehydrated medium for straightforward preparation.
3. Regulatory Compliance:
   • Meets APHA and ISO standards for water and wastewater testing.
4. Reliable Indicators:
   • Detects coliform presence with gas production and turbidity.

Warnings and Precautions

1. For use only by trained personnel in a laboratory setting.
2. Store in a dry environment and avoid contamination.
3. Dispose of waste in accordance with local and national regulations.

References

1. APHA (1980). Standard Methods for the Examination of Water and Wastewater, 15th ed.
2. Christen, G.L., et al. (1992). Coliforms and Other Indicator Bacteria.
3. ISO Standard 11866-2. Enumeration of Presumptive Escherichia coli in Milk and Milk Products.