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Middlebrook 7H11 + Drug is a solid, agar-based medium designed for susceptibility testing of Mycobacterium spp., particularly Mycobacterium tuberculosis. The medium contains specific anti-tuberculous drugs and is ideal for assessing drug resistance directly from clinical specimens. Compared to egg-based media like Lowenstein formulations, Middlebrook 7H11 demonstrates enhanced performance due to reduced drug inactivation.
Features and Benefits
- Agar-Based Medium:
- Promotes superior drug susceptibility testing compared to coagulated egg-based media.
- Enhanced viability and growth of M. tuberculosis, especially for fastidious strains.
- Comprehensive Composition:
- Nutrients: Pancreatic digest of casein, glutamic acid, and glucose provide nitrogen, vitamins, and energy.
- Selective Agents: Malachite green inhibits contaminants while protecting pH.
- Stabilizers: Catalase and albumin safeguard bacilli from toxic agents.
- Growth Enhancers: Oleic acid supports fatty acid metabolism.
- Specific Drug Inclusion:
- Available with various anti-tuberculous drugs at precise concentrations to assess resistance.
- Improved Diagnostic Accuracy:
- Enables clear interpretation of drug resistance in Mycobacterium tuberculosis complex (MTBC).
- Compliance with Standards:
- Recommended by CLSI and CDC for tuberculosis susceptibility testing.
Composition
| Component | Concentration (g/l or ml/l) |
|---|---|
| Pancreatic Digest of Casein | 1.0 |
| L-Glutamic Acid | 0.5 |
| Sodium Citrate | 0.4 |
| Pyridoxine Hydrochloride | 1.0 |
| Biotin | 0.0005 |
| Ferric Ammonium Citrate | 0.04 |
| Ammonium Sulfate | 0.5 |
| Disodium Phosphate | 1.5 |
| Monopotassium Phosphate | 1.5 |
| Magnesium Sulfate | 0.05 |
| Malachite Green | 1.0 |
| Agar | 15.0 |
| Glycerol | 5 ml |
| Sodium Chloride | 0.85 |
| Glucose | 2.0 |
| Bovine Albumin (Fraction V) | 5.0 |
| Catalase | 0.003 |
| Oleic Acid | 0.06 ml |
| Anti-Tuberculous Drug | Varies based on type |
| pH | 6.6 ± 0.2 |
Available Drug Formulations
| Drug Type | Concentration | Packaging (10 x 8.5 ml tubes) | Ref. |
|---|---|---|---|
| Amikacin | 2 µg/ml | Slant tubes | 37001 |
| Amikacin | 4 µg/ml | Slant tubes | 37002 |
| Ethambutol | 7.5 µg/ml | Slant tubes | 37006 |
| Ethionamide | 10 µg/ml | Slant tubes | 37011 |
| Isoniazid | 0.2 µg/ml | Slant tubes | 37016 |
| Rifampicin | 1 µg/ml | Slant tubes | 37041 |
(Refer to full product list in "Presentation" for additional formulations.)
Test Procedure
- Prepare Inoculum:
- Use freshly grown Mycobacterium tuberculosis from Middlebrook 7H9 Broth.
- Inoculate Media:
- Inoculate drug-free control (Middlebrook 7H11 Agar) alongside drug-containing media.
- Incubation:
- Shield plates and tubes from light.
- Incubate at 37 ± 1°C in 5-10% CO₂ for up to 3 weeks.
- Observation:
- Monitor weekly for growth and interpret results following CLSI/CDC guidelines.
Quality Control
| Microorganism | Expected Growth |
|---|---|
| Mycobacterium tuberculosis H37Ra | Inhibited |
Storage and Shelf Life
- Storage Conditions: 2-8°C, shielded from light.
- Shelf Life: 4 months from the date of production.
Applications
- Drug resistance testing of Mycobacterium tuberculosis.
- Research and clinical diagnostics in tuberculosis management.
- Differentiation of susceptible and resistant MTBC strains.
Ordering Information
| Product Description | Ref. | Packaging |
|---|---|---|
| Middlebrook 7H11 + Amikacin 2 µg/ml | 37001 | 10 x 8.5 ml tubes |
| Middlebrook 7H11 + Rifampicin 1 µg/ml | 37041 | 10 x 8.5 ml tubes |
| Middlebrook 7H11 + Ethambutol 7.5 µg/ml | 37006 | 10 x 8.5 ml tubes |
IFU: Middlebrook 7H11 + Amikacin
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Middlebrook 7H11 + Drug is a solid, agar-based medium designed for susceptibility testing of Mycobacterium spp., particularly Mycobacterium tuberculosis. The medium contains specific anti-tuberculous drugs and is ideal for assessing drug resistance directly from clinical specimens. Compared to egg-based media like Lowenstein formulations, Middlebrook 7H11 demonstrates enhanced performance due to reduced drug inactivation.
Features and Benefits
- Agar-Based Medium:
- Promotes superior drug susceptibility testing compared to coagulated egg-based media.
- Enhanced viability and growth of M. tuberculosis, especially for fastidious strains.
- Comprehensive Composition:
- Nutrients: Pancreatic digest of casein, glutamic acid, and glucose provide nitrogen, vitamins, and energy.
- Selective Agents: Malachite green inhibits contaminants while protecting pH.
- Stabilizers: Catalase and albumin safeguard bacilli from toxic agents.
- Growth Enhancers: Oleic acid supports fatty acid metabolism.
- Specific Drug Inclusion:
- Available with various anti-tuberculous drugs at precise concentrations to assess resistance.
- Improved Diagnostic Accuracy:
- Enables clear interpretation of drug resistance in Mycobacterium tuberculosis complex (MTBC).
- Compliance with Standards:
- Recommended by CLSI and CDC for tuberculosis susceptibility testing.
Composition
| Component | Concentration (g/l or ml/l) |
|---|---|
| Pancreatic Digest of Casein | 1.0 |
| L-Glutamic Acid | 0.5 |
| Sodium Citrate | 0.4 |
| Pyridoxine Hydrochloride | 1.0 |
| Biotin | 0.0005 |
| Ferric Ammonium Citrate | 0.04 |
| Ammonium Sulfate | 0.5 |
| Disodium Phosphate | 1.5 |
| Monopotassium Phosphate | 1.5 |
| Magnesium Sulfate | 0.05 |
| Malachite Green | 1.0 |
| Agar | 15.0 |
| Glycerol | 5 ml |
| Sodium Chloride | 0.85 |
| Glucose | 2.0 |
| Bovine Albumin (Fraction V) | 5.0 |
| Catalase | 0.003 |
| Oleic Acid | 0.06 ml |
| Anti-Tuberculous Drug | Varies based on type |
| pH | 6.6 ± 0.2 |
Available Drug Formulations
| Drug Type | Concentration | Packaging (10 x 8.5 ml tubes) | Ref. |
|---|---|---|---|
| Amikacin | 2 µg/ml | Slant tubes | 37001 |
| Amikacin | 4 µg/ml | Slant tubes | 37002 |
| Ethambutol | 7.5 µg/ml | Slant tubes | 37006 |
| Ethionamide | 10 µg/ml | Slant tubes | 37011 |
| Isoniazid | 0.2 µg/ml | Slant tubes | 37016 |
| Rifampicin | 1 µg/ml | Slant tubes | 37041 |
(Refer to full product list in "Presentation" for additional formulations.)
Test Procedure
- Prepare Inoculum:
- Use freshly grown Mycobacterium tuberculosis from Middlebrook 7H9 Broth.
- Inoculate Media:
- Inoculate drug-free control (Middlebrook 7H11 Agar) alongside drug-containing media.
- Incubation:
- Shield plates and tubes from light.
- Incubate at 37 ± 1°C in 5-10% CO₂ for up to 3 weeks.
- Observation:
- Monitor weekly for growth and interpret results following CLSI/CDC guidelines.
Quality Control
| Microorganism | Expected Growth |
|---|---|
| Mycobacterium tuberculosis H37Ra | Inhibited |
Storage and Shelf Life
- Storage Conditions: 2-8°C, shielded from light.
- Shelf Life: 4 months from the date of production.
Applications
- Drug resistance testing of Mycobacterium tuberculosis.
- Research and clinical diagnostics in tuberculosis management.
- Differentiation of susceptible and resistant MTBC strains.
Ordering Information
| Product Description | Ref. | Packaging |
|---|---|---|
| Middlebrook 7H11 + Amikacin 2 µg/ml | 37001 | 10 x 8.5 ml tubes |
| Middlebrook 7H11 + Rifampicin 1 µg/ml | 37041 | 10 x 8.5 ml tubes |
| Middlebrook 7H11 + Ethambutol 7.5 µg/ml | 37006 | 10 x 8.5 ml tubes |
IFU: Middlebrook 7H11 + Amikacin
This will display Shipping & Return.
Specifications
| Drug Concentration | Amikacin 2 µg/mL, Amikacin 4 µg/mL, Ethambutol 7.5 µg/mL, Ethionamide 10 µg/mL, Isoniazid 0.2 µg/mL, Isoniazid 1 µg/mL, Kanamicin 6 µg/mL, p-Aminosalycilic acid 8 µg/mL, Pyrazinamide 25 µg/mL, Rifabutin 1 µg/mL, Rifampicin 1 µg/mL, Streptomycin 2 µg/mL, Ofloxacin 2 µg/mL, Cycloserine 30ug/mL |