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Invitrogen™ Novex™ Sharp Pre-stained Protein Standard, 2 x 250 µL
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enough for 50 lanes at 10 µL per application
Specifications:
| Application | Protein Electrophoresis & Western Blotting | ||
| Storage Temperature | -20°C | ||
| Product Type | Protein Standards | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™, Invitrogen | ||
| Product Grade | Molecular Biology | ||
The Invitrogen™ Novex™ Sharp Pre-Stained Protein Standard is a ready-to-use, multicolored protein molecular weight marker designed for accurate sizing and monitoring of protein migration in SDS-PAGE and Western blotting applications. It consists of 12 well-defined, pre-stained bands that span a wide molecular weight range from 3.5 kDa to 260 kDa, allowing users to verify protein size, monitor gel run progress, and confirm transfer efficiency onto membranes.
Each band is colored (blue, pink, or yellow) to ensure easy visualization and orientation during electrophoresis and blotting procedures. The ladder is premixed with loading buffer, requiring no heating, dilution, or additional reagents prior to use—simply thaw and load.
Key Features and Benefits
- Wide Molecular Weight Coverage: 12 pre-stained protein bands from 3.5 to 260 kDa
- Multicolor Visualization: Includes 3 distinct colors (blue, pink, yellow) for quick orientation
- Ready-to-Use Format: No preparation, dilution, or heating required—load directly onto gel
- High Clarity and Resolution: Sharp, bright bands support precise sizing and tracking
- Versatile Compatibility: Compatible with Tris-Glycine, Tricine, and Bis-Tris gel systems
- Convenient Storage: Long shelf life when stored at –20°C; shipped on wet or dry ice
Applications
- Monitoring protein migration during SDS-PAGE
- Verifying transfer efficiency during Western blotting
- Estimating the molecular weight of unknown proteins
- Teaching and demonstration of protein electrophoresis
- Routine quality control in protein expression labs
Specifications
| Attribute | Details |
|---|---|
| Molecular Weight Range | 3.5 to 260 kDa |
| Number of Bands | 12 |
| Stain Type | Multicolored: Blue, Pink, Yellow |
| Detection Method | Colorimetric (visible by eye) |
| Gel Compatibility | Tris-Glycine, Tricine, NuPAGE Bis-Tris, Bolt |
| Format | Ready-to-load |
| Volume | 2 × 250 μL |
| Loading Volume | 10 µL per lane recommended |
| Storage Conditions | Store at –20°C |
| Shipping Conditions | Approved for shipment on wet or dry ice |
| Buffer Composition | 65 mM Tris (pH 6.5), 30% glycerol, 2% SDS, 2.5 mM EDTA |
Kit Contents
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2 × 250 µL of Novex™ Sharp Pre-Stained Protein Standard
(enough for 50 lanes at 10 µL per application)
The Novex™ Sharp Pre-Stained Protein Standard provides researchers with a reliable and easy-to-use solution for estimating protein molecular weight and verifying transfer success. With its color-coded, sharp bands and broad size range, it is ideal for routine protein gel electrophoresis and blotting workflows, offering clarity, convenience, and confidence in every experiment.