Invitrogen™ UltraComp eBeads™ Compensation Beads

Universal Single-Color Compensation Beads for Mouse, Rat, and Hamster Antibodies in Multicolor Flow Cytometry

UltraComp eBeads™ Compensation Beads are high-performance, ready-to-use beads designed for use as single-color compensation controls in multicolor flow cytometry experiments. These beads bind fluorochrome-conjugated antibodies from mouse, rat, or hamster origin, regardless of immunoglobulin light chain, enabling standardized and reproducible compensation setup across a wide range of laser lines.

Each drop of UltraComp eBeads contains a positive and negative population, producing a bimodal fluorescence signal after antibody staining—ideal for defining compensation values across detectors in high-parameter panels.

Key Features and Benefits

• Species Flexibility
  Binds mouse, rat, and hamster antibodies, regardless of light chain specificity.
• Compatible with All Laser Lines
  Designed for use with fluorochromes excited by UV (355 nm), violet (405 nm), blue (488 nm), green (532 nm), yellow-green (561 nm), and red (633–640 nm) lasers.
• Bimodal Fluorescence Signal
  Each drop contains a positive and negative bead population, simplifying compensation setup by providing distinct signal separation.
• Universal Use Across Panels
  Works with all fluorochrome-conjugated antibodies, including tandem dyes, making it suitable for complex flow cytometry experiments.
• Convenient and Stable Format
  Provided in a dropper bottle format; stable and ready to use without additional preparation.

Applications

• Single-color compensation controls for multicolor flow cytometry
• Standardization of fluorescence compensation for mouse, rat, or hamster antibody panels
• Quality control in immune profiling, T/B cell assays, and intracellular staining workflows

Technical Specifications

Invitrogen™ UltraComp eBeads™ Compensation Beads offer a robust, flexible, and validated solution for standardizing single-color compensation controls in multicolor flow cytometry. Their compatibility with a wide range of fluorochromes and laser lines makes them an essential tool for any researcher conducting high-parameter immune profiling or complex antibody panel optimization.