Invitrogen™ Platinum™ PCR SuperMix High Fidelity

The Invitrogen™ Platinum™ PCR SuperMix High Fidelity is a ready-to-use master mix that streamlines the PCR setup process while providing high-specificity, high-fidelity DNA amplification. This advanced formulation combines Platinum™ Taq DNA Polymerase High Fidelity with a proprietary blend of salts, magnesium, and dNTPs, requiring only the addition of primers and template DNA.

The unique enzyme mix—featuring Taq DNA polymerase and a proofreading Pyrococcus species GB-D polymerase—delivers up to six times greater fidelity than standard Taq polymerase, ensuring accurate amplification of DNA fragments up to 15 kb. Built-in anti-Taq antibodies enable automatic hot-start PCR, enhancing specificity and yield while allowing reaction setup at room temperature.

Key Features

• High Fidelity Amplification: Provides up to 6× greater accuracy than standard Taq DNA polymerase.
• Hot-Start Technology: Anti-Taq antibodies prevent non-specific amplification, improving yield and specificity.
• Long Fragment Capability: Efficiently amplifies DNA fragments up to 15 kb in length.
• Convenient SuperMix Format: Ready-to-use formulation minimizes setup time and reduces pipetting errors.
• Reliable Performance: Consistent amplification across a broad range of templates and GC content.
• Room Temperature Setup: Enables easy and contamination-free reaction assembly without ice.

Specifications

Applications

• High-fidelity DNA amplification for cloning and sequencing
• Site-directed mutagenesis requiring proofreading accuracy
• PCR amplification of long DNA fragments (≤15 kb)
• Routine hot-start PCR with reduced nonspecific amplification
• Research protocols demanding reliable and reproducible amplification

The Invitrogen™ Platinum™ PCR SuperMix High Fidelity provides researchers with a powerful, convenient, and accurate PCR solution. By integrating proofreading polymerase technology with built-in hot-start capability, this SuperMix ensures reliable amplification, superior fidelity, and simplified workflow—ideal for cloning, mutagenesis, and other applications requiring precise DNA synthesis.