Invitrogen™ eBioscience™ IL-17A Monoclonal Antibody (eBio64DEC17), Brilliant Violet™ 605

The Invitrogen™ IL-17A Monoclonal Antibody (eBio64DEC17), conjugated to Brilliant Violet™ 605, is a high-performance antibody designed for the detection of human IL-17A. IL-17A is a pro-inflammatory cytokine secreted by Th17 cells and plays a crucial role in inflammation, autoimmunity, and host defense. This antibody is validated for intracellular staining in flow cytometry, providing bright fluorescence for clear identification of IL-17A-expressing cells.

Key Features:

1. Target and Specificity:
   • Recognizes human IL-17A, a cytokine involved in promoting inflammation and regulating immune responses.
   • High specificity ensures precise detection in immune cell studies.
2. Clone and Conjugate:
   • Clone: eBio64DEC17, a trusted clone known for reliable performance.
   • Conjugate: Brilliant Violet™ 605, a tandem dye providing bright fluorescence with excitation at 407 nm and emission at 605 nm.
3. Validated Applications:
   • Optimized for flow cytometry, enabling robust analysis of IL-17A expression in human immune cells.
4. Bright and Stable Fluorescence:
   • Brilliant Violet™ 605 delivers high-intensity signals with minimal background, making it ideal for multicolor flow cytometry panels.
5. Pre-Titrated and Ready-to-Use:
   • Requires only 5 µL (0.25 µg) per test, simplifying experimental workflows.

Specifications:

Applications:

1. Flow Cytometry:
   • Detect intracellular IL-17A expression in Th17-polarized cells and other immune cell populations.
   • Ideal for multicolor panels using a violet laser-equipped flow cytometer.
2. Th17 Research:
   • Analyze IL-17A production in Th17 cells under IL-23-dependent polarization conditions.
3. Inflammation Studies:
   • Study IL-17A’s role in inflammatory diseases, including rheumatoid arthritis, psoriasis, and asthma.
4. Cytokine Profiling:
   • Use in immune regulation and autoimmune disease research to investigate IL-17A pathways.

Key Benefits:

1. Bright and Stable Fluorescence:
   • Brilliant Violet™ 605 provides high-intensity, photostable signals for clear separation of IL-17A-positive and negative populations.
2. Reproducible Results:
   • Pre-titrated formulation ensures consistency and reliability across experiments.
3. Flexible and User-Friendly:
   • Compatible with a variety of fixation and permeabilization buffers for intracellular staining.
4. Optimized for Multicolor Panels:
   • Minimal spectral overlap enhances compatibility with other fluorochromes in complex flow cytometry panels.

Usage Recommendations:

1. Sample Preparation:
   • Polarize human peripheral blood CD4+ T cells under Th17 conditions with IL-23 or similar reagents.
2. Staining Protocol:
   • Use the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) for optimal intracellular staining.
   • Add 5 µL (0.25 µg) of antibody per 100 µL of cell suspension.
3. Flow Cytometry:
   • Analyze using a flow cytometer equipped with a violet laser (405 nm).
4. Fixation:
   • Fix samples with appropriate buffers to preserve fluorescence for up to 3 days.
5. Staining Panels:
   • Use Brilliant Stain Buffer™ to minimize non-specific interactions when combining multiple polymer dyes.

Storage and Handling:

• Store at 4°C in the dark to protect the Brilliant Violet™ 605 fluorochrome.
• Avoid freezing, as freezing may degrade the antibody or fluorochrome.

Target Information:

• IL-17A:
  • A pro-inflammatory cytokine secreted by Th17 cells, critical in immune regulation and host defense.
  • Plays a significant role in inflammatory diseases, such as rheumatoid arthritis, psoriasis, and transplant rejection.
  • Stimulates fibroblasts and epithelial cells to secrete cytokines like IL-6, IL-8, and G-CSF, amplifying inflammatory responses.

For Research Use Only:

This product is intended for research purposes only and is not for diagnostic or therapeutic applications.