Invitrogen™ eBioscience™ 1-step Fix/Lyse Solution (10X)

The Invitrogen™ eBioscience™ 1-step Fix/Lyse Solution (10X) is a specialized reagent designed to simplify the preparation of peripheral blood cells for flow cytometry analysis. This all-in-one solution allows for simultaneous lysis of red blood cells (RBCs) and fixation of leukocytes after staining with fluorochrome-conjugated antibodies. By eliminating the need for gradient centrifugation methods like Ficoll-Paque density separation, it streamlines the sample preparation process and ensures high-quality results.

Key Features:

1. Red Blood Cell Lysis:
   • Effectively lyses non-nucleated erythrocytes while preserving leukocyte integrity and fluorochrome-labeled markers.
2. Fixation and Compatibility:
   • Fixes leukocytes during the lysis step, stabilizing stained cells for flow cytometry.
   • Compatible with organic dyes and tandem dye-conjugated antibodies, ensuring flexibility in multicolor flow cytometry panels.
3. Simplified Workflow:
   • Eliminates the need for mononuclear cell isolation via gradient centrifugation, saving time and effort.
4. 10X Concentration:
   • Supplied as a concentrated formulation, providing convenience and flexibility in preparing the working solution.
5. Ready-to-Use:
   • Designed for ease of use with simple protocols that integrate seamlessly into existing workflows.

Specifications:

Applications:

1. Flow Cytometry:
   • Prepares whole blood samples for flow cytometry by lysing RBCs and fixing leukocytes in a single step.
2. Multicolor Staining Panels:
   • Compatible with fluorochrome-conjugated antibodies, including organic and tandem dyes, for advanced immunophenotyping.
3. Streamlined Sample Preparation:
   • Ideal for laboratories requiring quick and efficient preparation of peripheral blood samples without density gradient centrifugation.

Key Benefits:

1. Efficiency:
   • Combines red blood cell lysis and leukocyte fixation into a single step, reducing sample processing time.
2. Preserves Fluorescence:
   • Maintains the integrity of fluorochrome-conjugated antibodies, ensuring bright and reliable signals during flow cytometry.
3. User-Friendly Protocol:
   • Simplifies workflow by removing the need for additional cell isolation techniques like Ficoll-Paque separation.
4. Versatility:
   • Compatible with a wide range of fluorochrome-conjugated antibodies, enabling flexibility in experimental design.

Protocol Highlights:

1. Staining:
   • Stain peripheral blood cells with fluorochrome-conjugated antibodies.
2. Fix and Lyse:
   • Add the 1-step Fix/Lyse Solution to the stained cells to lyse erythrocytes and fix leukocytes.
3. Washing:
   • Wash the cells after the fixation/lysis step, then analyze by flow cytometry.

Storage and Handling:

• Store at 2–8°C to maintain stability.
• Do not freeze the solution to prevent degradation of its components.

For Research Use Only:

This product is intended for research purposes only and is not suitable for diagnostic or therapeutic applications.