Invitrogen™ CDP-Star™ Substrate (12.5 mM Concentrate)

Concentrated, high-sensitivity chemiluminescent substrate for alkaline phosphatase detection

The Invitrogen™ CDP-Star™ Substrate (12.5 mM Concentrate) is a high-performance chemiluminescent substrate for alkaline phosphatase (AP), delivering exceptional sensitivity, rapid signal generation, and long-lasting glow emission. Suitable for both membrane-based and solution-based assays, this substrate enables clear detection of AP and AP-labeled molecules with superior signal-to-noise ratio and low background luminescence.

Optimized for applications including Southern, Northern, and Western blotting, as well as immunoassays, DNA probe assays, enzyme assays, and reporter gene assays, CDP-Star™ achieves maximum light levels in approximately 10 minutes, with emission persisting for several hours—providing flexibility for multiple exposures and reimaging.

Key Features & Benefits

• High Sensitivity – Detects alkaline phosphatase labels with superior intensity and clarity.
• Fast Signal Development – Peak chemiluminescence in ~10 minutes.
• Long-Lasting Glow – Stable light emission for several hours.
• Low Background – Produces clean, high-contrast results.
• Versatile – Suitable for blotting and solution-based enzymatic detection assays.
• Concentrated Format – Allows custom dilution to suit application needs.

Applications

• Southern Blotting – DNA detection with AP-labeled probes.
• Northern Blotting – RNA detection in gene expression studies.
• Western Blotting – Protein detection using AP-conjugated antibodies.
• Solution-Based Assays – Immunoassays, DNA probe assays, enzyme assays, and reporter gene assays.

Technical Specifications

Ordering Information

The Invitrogen™ CDP-Star™ Substrate (12.5 mM Concentrate) provides superior sensitivity, speed, and signal stability for AP-based detection methods. Its concentrated format offers flexibility for various assay types, making it a versatile choice for high-performance molecular biology and immunodetection applications.