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The Invitrogen™ CD32 Monoclonal Antibody (6C4), APC, eBioscience™ is a high-quality antibody designed for flow cytometry applications, specifically targeting human CD32 (Fc gammaRII). This APC-conjugated monoclonal antibody is optimized for detecting CD32 isoforms Fc gammaRIIA and Fc gammaRIIB, which are expressed on B cells, monocytes, granulocytes, macrophages, and platelets.
CD32 plays a crucial role in immune complex clearance, inflammation, and autoimmune disease mechanisms. This antibody is validated for high-specificity binding and is ideal for immunophenotyping, immune cell characterization, and functional studies in research.
Key Features & Benefits
1. High-Specificity Detection of CD32 (Fc gammaRII)
- Recognizes CD32A and CD32B isoforms, ensuring comprehensive CD32 profiling.
- Suitable for studying immune responses, Fc receptor interactions, and phagocytosis pathways.
- Tested and validated for flow cytometry, providing clear and reproducible staining results.
2. APC Conjugation for Enhanced Fluorescence
- Conjugated to Allophycocyanin (APC) for strong red fluorescence (Excitation: 651 nm, Emission: 660 nm).
- Compatible with multi-color flow cytometry panels using a red laser (633-647 nm).
- Ensures minimal spectral overlap, enhancing data accuracy in complex immunophenotyping experiments.
3. Ready-to-Use for Flow Cytometry Applications
- Pre-titrated for optimal staining at 5 µL (0.5 µg)/test.
- Filtered through a 0.2 µm membrane for maximum purity and batch-to-batch consistency.
- Supplied in PBS buffer (pH 7.2) with 0.2% BSA and 0.09% sodium azide for long-term stability.
4. Highly Reproducible & Published in Research
- Validated in peer-reviewed studies, demonstrating reliable performance.
- Used in immunology, inflammation, autoimmune disease, and infectious disease research.
Product Specifications
| Property | Details |
|---|---|
| Brand | Invitrogen™ eBioscience™ |
| Antibody Target | CD32 (Fc gammaRII) |
| Clone | 6C4 |
| Isotype | Mouse IgG1, kappa |
| Conjugate | APC (Allophycocyanin) |
| Excitation/Emission Max | 651/660 nm (Red Laser) |
| Application | Flow Cytometry |
| Species Reactivity | Human |
| Published Reactivity | Human, Rhesus monkey |
| Form | Liquid |
| Concentration | 5 µL/test |
| Purification Method | Affinity Chromatography |
| Storage Conditions | 4°C, Store in Dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic), Wet Ice (international) |
| RRID | AB_1272032 |
Available Variants & Ordering Information
| Catalog Number | Unit Size |
|---|---|
| 17-0329-42 | 100 Tests |
| 17-0329-41 | 25 Tests |
Other Formats Available:
| Catalog Number | Conjugate | Unit Size |
|---|---|---|
| 35-0329-42 | PE-Cyanine5.5 | 100 Tests |
| 16-0329-81 | Functional Grade | 50 µg |
| 46-0329-42 | PerCP-eFluor™ 710 | 100 Tests |
| 12-0329-42 | PE | 100 Tests |
| 25-0329-42 | PE-Cyanine7 | 100 Tests |
| 48-0329-42 | eFluor™ 450 | 100 Tests |
| 11-0329-42 | FITC | 100 Tests |
| 13-0329-82 | Biotin | 100 Tests |
Use Cases & Applications
1. Immunophenotyping & Flow Cytometry
- Characterization of immune cell populations expressing CD32.
- Multi-color flow cytometry panels for studying Fc receptor interactions.
2. Inflammation & Autoimmune Disease Research
- Investigating CD32’s role in immune complex recognition and clearance.
- Studying Fc receptor involvement in autoimmune conditions.
3. Infectious Disease & Immunology Studies
- Assessing monocyte and granulocyte responses to infections.
- Exploring natural killer (NK) cell interactions with opsonized antigens.
Published Research & References
- Cytometry Part A (2018): Application of CD32 antibody in antibody-dependent cell-mediated cytotoxicity (ADCC) assays.
- Mediators of Inflammation (2017): Role of CD32 in autoimmune disease research.
- Journal of Neurovirology (2017): CD32 detection in microglial cells related to HIV research.
The Invitrogen™ CD32 Monoclonal Antibody (6C4), APC, eBioscience™ is a high-specificity reagent for flow cytometry-based immune cell characterization. Featuring APC conjugation for bright fluorescence, validated performance, and minimal spectral overlap, this antibody is an ideal choice for immunophenotyping, Fc receptor studies, and autoimmune research.
CD32 Monoclonal Antibody
The Invitrogen™ CD32 Monoclonal Antibody (6C4), APC, eBioscience™ is a high-quality antibody designed for flow cytometry applications, specifically targeting human CD32 (Fc gammaRII). This APC-conjugated monoclonal antibody is optimized for detecting CD32 isoforms Fc gammaRIIA and Fc gammaRIIB, which are expressed on B cells, monocytes, granulocytes, macrophages, and platelets.
CD32 plays a crucial role in immune complex clearance, inflammation, and autoimmune disease mechanisms. This antibody is validated for high-specificity binding and is ideal for immunophenotyping, immune cell characterization, and functional studies in research.
Key Features & Benefits
1. High-Specificity Detection of CD32 (Fc gammaRII)
- Recognizes CD32A and CD32B isoforms, ensuring comprehensive CD32 profiling.
- Suitable for studying immune responses, Fc receptor interactions, and phagocytosis pathways.
- Tested and validated for flow cytometry, providing clear and reproducible staining results.
2. APC Conjugation for Enhanced Fluorescence
- Conjugated to Allophycocyanin (APC) for strong red fluorescence (Excitation: 651 nm, Emission: 660 nm).
- Compatible with multi-color flow cytometry panels using a red laser (633-647 nm).
- Ensures minimal spectral overlap, enhancing data accuracy in complex immunophenotyping experiments.
3. Ready-to-Use for Flow Cytometry Applications
- Pre-titrated for optimal staining at 5 µL (0.5 µg)/test.
- Filtered through a 0.2 µm membrane for maximum purity and batch-to-batch consistency.
- Supplied in PBS buffer (pH 7.2) with 0.2% BSA and 0.09% sodium azide for long-term stability.
4. Highly Reproducible & Published in Research
- Validated in peer-reviewed studies, demonstrating reliable performance.
- Used in immunology, inflammation, autoimmune disease, and infectious disease research.
Product Specifications
| Property | Details |
|---|---|
| Brand | Invitrogen™ eBioscience™ |
| Antibody Target | CD32 (Fc gammaRII) |
| Clone | 6C4 |
| Isotype | Mouse IgG1, kappa |
| Conjugate | APC (Allophycocyanin) |
| Excitation/Emission Max | 651/660 nm (Red Laser) |
| Application | Flow Cytometry |
| Species Reactivity | Human |
| Published Reactivity | Human, Rhesus monkey |
| Form | Liquid |
| Concentration | 5 µL/test |
| Purification Method | Affinity Chromatography |
| Storage Conditions | 4°C, Store in Dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic), Wet Ice (international) |
| RRID | AB_1272032 |
Available Variants & Ordering Information
| Catalog Number | Unit Size |
|---|---|
| 17-0329-42 | 100 Tests |
| 17-0329-41 | 25 Tests |
Other Formats Available:
| Catalog Number | Conjugate | Unit Size |
|---|---|---|
| 35-0329-42 | PE-Cyanine5.5 | 100 Tests |
| 16-0329-81 | Functional Grade | 50 µg |
| 46-0329-42 | PerCP-eFluor™ 710 | 100 Tests |
| 12-0329-42 | PE | 100 Tests |
| 25-0329-42 | PE-Cyanine7 | 100 Tests |
| 48-0329-42 | eFluor™ 450 | 100 Tests |
| 11-0329-42 | FITC | 100 Tests |
| 13-0329-82 | Biotin | 100 Tests |
Use Cases & Applications
1. Immunophenotyping & Flow Cytometry
- Characterization of immune cell populations expressing CD32.
- Multi-color flow cytometry panels for studying Fc receptor interactions.
2. Inflammation & Autoimmune Disease Research
- Investigating CD32’s role in immune complex recognition and clearance.
- Studying Fc receptor involvement in autoimmune conditions.
3. Infectious Disease & Immunology Studies
- Assessing monocyte and granulocyte responses to infections.
- Exploring natural killer (NK) cell interactions with opsonized antigens.
Published Research & References
- Cytometry Part A (2018): Application of CD32 antibody in antibody-dependent cell-mediated cytotoxicity (ADCC) assays.
- Mediators of Inflammation (2017): Role of CD32 in autoimmune disease research.
- Journal of Neurovirology (2017): CD32 detection in microglial cells related to HIV research.
The Invitrogen™ CD32 Monoclonal Antibody (6C4), APC, eBioscience™ is a high-specificity reagent for flow cytometry-based immune cell characterization. Featuring APC conjugation for bright fluorescence, validated performance, and minimal spectral overlap, this antibody is an ideal choice for immunophenotyping, Fc receptor studies, and autoimmune research.
CD32 Monoclonal Antibody
Specifications
| Pack Size | For 100 Tests, for 25 Tests |