Invitrogen™ TNF alpha Human ELISA Kit, 96 Tests

The Invitrogen™ Human TNF Alpha ELISA Kit (KHC3011) is a high-sensitivity, sandwich ELISA assay designed for quantifying TNF-alpha in human serum, plasma, and cell culture supernatants. This kit provides reliable, reproducible results with an analytical sensitivity of 1.7 pg/mL, making it ideal for immunology, inflammation, and cytokine research.

Featuring pre-coated 96-well plates and ready-to-use reagents, this ELISA kit ensures efficient workflow execution, with a total time-to-result of just 4 hours. Each lot is rigorously validated for sensitivity, specificity, and lot-to-lot consistency, providing researchers with high-confidence data.

Key Features & Benefits

1. High Sensitivity & Wide Dynamic Range

• Analytical sensitivity: 1.7 pg/mL ensures detection of low-abundance TNF-alpha.
• Assay range: 15.6 – 1000 pg/mL, allowing for quantification across a broad sample range.

2. Fast & Efficient Workflow

• Total assay time of 4 hours with only 1 hour 20 minutes hands-on time.
• Ready-to-use reagents and pre-coated microplate streamline the workflow, reducing preparation time.

3. Reliable & Reproducible Performance

• Low interassay CV (7.5%) and intraassay CV (4.4%) ensure high precision.
• Validated for human serum, plasma, and supernatant samples.

4. Robust & High-Specificity Sandwich ELISA Format

• Capture antibody pre-coated onto microplate wells ensures high binding affinity for TNF-alpha.
• Detection antibody conjugated to biotin, with HRP-streptavidin for signal amplification.
• Colorimetric detection via TMB substrate, readable on standard microplate readers.

5. Comprehensive Kit Components for Easy Setup

• Includes all necessary reagents:
  • Pre-coated 96-well plate
  • Standard dilution buffer & TNF-alpha standard
  • Biotinylated detection antibody & Streptavidin-HRP
  • HRP diluent, wash buffer, chromogen, and stop solution
  • Adhesive plate covers for sample protection

Specifications

Use Cases & Applications

1. Immunology & Inflammation Research

• Quantifies TNF-alpha cytokine levels in immune responses.
• Ideal for studying inflammatory pathways in autoimmune diseases.
• Supports research on rheumatoid arthritis, lupus, and inflammatory bowel disease (IBD).

2. Infectious Disease & Cytokine Profiling

• Evaluates TNF-alpha response to bacterial, viral, and parasitic infections.
• Essential for understanding sepsis, COVID-19 immune response, and other inflammatory conditions.

3. Cancer & Tumor Microenvironment Studies

• Measures TNF-alpha involvement in tumor necrosis and apoptosis.
• Helps identify pro-inflammatory cytokine roles in tumor progression and immune evasion.

4. Drug Development & Biomarker Discovery

• Used in preclinical testing of anti-inflammatory drugs and TNF inhibitors.
• Facilitates cytokine biomarker identification for targeted immunotherapy research.

Ordering Information

Target Information: TNF Alpha (Tumor Necrosis Factor Alpha)

TNF alpha is a proinflammatory cytokine belonging to the TNF superfamily, primarily secreted by macrophages. It plays a pivotal role in immune regulation, apoptosis, and inflammation, binding to TNFR1 (TNFRSF1A) and TNFR2 (TNFRSF1B) receptors. TNF alpha is associated with autoimmune disorders, metabolic diseases, and tumor necrosis mechanisms, making it a key target in immunology, oncology, and infectious disease research.

Aliases:

• TNF-a, TNFSF2, Cachectin, DIF, TNF ligand 1F

UniProt ID (Human): P01375

The Invitrogen™ Human TNF Alpha ELISA Kit (KHC3011) provides a high-sensitivity, validated assay for TNF-alpha quantification in human samples. With a broad dynamic range (15.6 – 1000 pg/mL), reproducible results, and a user-friendly workflow, this kit is an essential tool for immunology, inflammation, and biomarker research.