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Invitrogen™ IL-4 Monoclonal Antibody (11B11), PE, eBioscience™
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Specifications:
| Application | Flow Cytometry | ||
| Storage Temperature | 2-8°C | ||
| Product Type | antibody | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™, Invitrogen | ||
| Product Grade | Molecular Biology | ||
Rat Anti-Mouse IL-4 | PE-Conjugated Antibody for Intracellular Cytokine Detection by Flow Cytometry
The Invitrogen™ IL-4 Monoclonal Antibody (Clone 11B11) conjugated to Phycoerythrin (PE) is a validated, high-performance reagent for the intracellular detection of mouse interleukin-4 (IL-4). IL-4 is a key Th2 cytokine that regulates B cell proliferation, isotype switching, and upregulation of MHC class II molecules.
This antibody is widely cited for flow cytometry applications and has been used in over 75 publications for analyzing IL-4 expression in mouse immune cells.
Key Features and Benefits
- Clone 11B11: A neutralizing monoclonal antibody against murine IL-4, a 14 kDa cytokine.
- PE Conjugation: Fluorescent emission peak at 578 nm, ideal for use with blue, green, or yellow-green lasers.
- Intracellular Staining: Validated for intracellular flow cytometry of Th2-polarized mouse splenocytes.
- Highly Cited: Supported by 78+ peer-reviewed publications and compatible with standard ICS workflows.
Applications
- Flow Cytometry (Tested): ≤0.125 µg/test (in 100 µL final volume)
- Intracellular Cytokine Staining (ICS)
- Th2 Response Characterization
- Cytokine Profiling in Mouse Models
Optimize staining concentration for cell number and experimental conditions.
Technical Specifications
| Property | Details |
|---|---|
| Clone | 11B11 |
| Host / Isotype | Rat / IgG1, kappa |
| Conjugate | Phycoerythrin (PE) |
| Excitation / Emission Max | 565 / 576 nm (Peak Emission ~578 nm) |
| Species Reactivity | Mouse |
| Published Reactivity | Mouse, Rat |
| Tested Application | Flow Cytometry (Intracellular) |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 with 0.09% sodium azide |
| Storage Conditions | 4°C, protect from light, do not freeze |
| Shipping Conditions | Ambient (domestic), Wet ice (international) |
| Isotype Control Recommended | Rat IgG1 kappa Isotype Control (eBRG1), PE |
| RRID | AB_466156 |
| Filtration | 0.2 µm post-manufacturing filtered |
Available Pack Sizes
| Catalog Number | Quantity | Format |
|---|---|---|
| 12-7041-81 | 50 µg | Liquid |
| 12-7041-82 | 100 µg | Liquid |
Target Information – IL-4
Interleukin-4 (IL-4) is a pleiotropic cytokine produced predominantly by Th2 cells, NK T cells, and mast cells. It promotes B cell class switching, IgE production, and plays a pivotal role in humoral immunity and allergic inflammation. IL-4 acts via the IL-4 receptor, which also binds IL-13, leading to overlapping functional roles. The IL-4 gene is part of a cytokine cluster located on mouse chromosome 11 and human chromosome 5q.
Invitrogen™ IL-4 Monoclonal Antibody (11B11), PE is a reliable, peer-reviewed reagent designed for intracellular detection of IL-4 in mouse immune cells. With excellent performance in flow cytometry, it is ideal for studies involving T helper 2 cell responses, cytokine profiling, and inflammatory models.
- Pack Size: 50 µg 100 µg