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Invitrogen™ Human Luteinizing Hormone ELISA Kit
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Specifications:
| Application | ELISA-Based Assays | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Elisa Kit | Forms | Kit with Various components |
| Product Brand | Thermo Fisher Scientific™, Invitrogen | ||
| Product Grade | Molecular Biology | ||
The Invitrogen™ Human Luteinizing Hormone (LH) ELISA Kit is a quantitative sandwich enzyme-linked immunosorbent assay for the detection and measurement of human luteinizing hormone (LH) in serum, plasma, and cell culture supernatants. Optimized for research use only, this kit detects both natural and recombinant LH with high sensitivity and reproducibility, making it ideal for reproductive biology and endocrine research.
Assay Principle
This solid-phase sandwich ELISA uses:
- Pre-coated microplate wells with a target-specific capture antibody.
- Biotinylated detection antibody for high specificity.
- Streptavidin-HRP conjugate for signal amplification.
- A chromogenic HRP substrate to produce a measurable color change proportional to LH concentration.
Key Features
- High Sensitivity – Detects as low as 140 pg/mL.
- Wide Dynamic Range – 156.3–10,000 pg/mL.
- Small Sample Volume – 50 μL for serum/plasma, 100 μL for supernatants.
- Reliable Reproducibility – Intra-assay CV <10%, Inter-assay CV <12%.
- Validated Performance – Each lot tested for sensitivity, specificity, and consistency.
Applications
- Quantitative measurement of LH in human serum, plasma, or cell culture media.
- Endocrinology research focused on reproductive hormone regulation.
- Fertility and ovulation studies in human models.
- Investigation of hypogonadism and other LH-related disorders.
- Drug screening for modulators of reproductive hormone pathways.
Technical Specifications
| Parameter | Specification |
|---|---|
| Target Protein | Luteinizing Hormone (LH) |
| Species Reactivity | Human |
| Assay Type | Sandwich ELISA |
| Sensitivity | 140 pg/mL |
| Assay Range | 156.3–10,000 pg/mL |
| Sample Types | Serum, Plasma, Cell Culture Supernatants |
| Sample Volume | 50 μL (serum/plasma), 100 μL (supernatant) |
| Intra-assay CV | <10% |
| Inter-assay CV | <12% |
| Assay Time | ~4 hr 45 min (1 hr 20 min hands-on) |
| Detection | Biotin-labeled antibody + Streptavidin-HRP |
| Readout | Absorbance (microplate reader) |
| Storage | 2–8°C (short term), –20°C (long term) |
Kit Contents
- Pre-coated 96-well plate
- Human LH standard
- Assay diluent concentrate
- Biotinylated detection antibody
- Streptavidin-HRP conjugate
- Wash buffer
- Chromogen substrate
- Stop solution
- Adhesive plate covers
The Invitrogen™ Human LH ELISA Kit provides a high-performance, reproducible, and sensitive method for luteinizing hormone quantification in various human sample types. With rigorous lot validation and a wide detection range, it is an essential tool for reproductive endocrinology, fertility research, and hormone pathway studies.
- Pack Size: 10 x 96 Tests Plate 96 Tests Plate 5 x 96 Tests Plates