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Invitrogen™ Fixative-Free Lysing Solution, High-Yield Lyse, 500mL
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Specifications:
| Application | Cell culture, Flow Cytometry | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Buffer | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™, Invitrogen | ||
| Product Grade | Molecular Biology | ||
Fixative-Free, High-Yield Erythrocyte Lysis for Whole Blood Flow Cytometry
Invitrogen™ High-Yield Lyse is a premixed, fixative-free erythrocyte lysing solution designed to efficiently remove red blood cells from whole blood samples while preserving the integrity and viability of rare leukocyte populations. This reagent enables post-staining lysis, making it ideal for use with fluorochrome-conjugated antibodies in flow cytometric analysis.
Unlike fixative-based lysing reagents, High-Yield Lyse preserves surface antigen expression, supporting accurate and reproducible immunophenotyping without additional permeabilization or fixation steps.
Key Features and Benefits
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Fixative-Free Formulation
Enables gentle erythrocyte removal without fixing cells, preserving native marker expression and cell viability. -
High Recovery of Rare Populations
Minimizes leukocyte loss, making it ideal for rare cell detection, such as stem cells or activated lymphocytes. -
Post-Staining Lysis Compatible
Designed for use after antibody staining, eliminating the need for pre-lysis and simplifying multicolor flow cytometry workflows. -
Convenient, Ready-to-Use Format
Premixed and supplied as a 1X working solution—no dilution or additional reagents required.
Applications
- Flow cytometry sample preparation from whole blood
- Leukocyte immunophenotyping
- Rare event detection and analysis
- Surface marker analysis without fixation artifacts
- Pre-sorting cleanup for FACS workflows
Technical Specifications
| Property | Details |
|---|---|
| Product Name | Fixative-Free Lysing Solution, High-Yield Lyse |
| Catalog Number | HYL250 |
| Volume | 500 mL |
| Format | Liquid, ready-to-use |
| Lysis Target | Erythrocytes (Red Blood Cells) |
| Sample Type | Whole blood |
| Reagent Type | Whole blood lysis buffer |
| Fixative-Free | Yes |
| Shelf Life | 24 months from date of manufacture |
| Storage Conditions | Room temperature |
| Shipping Conditions | Ambient |
| Product Type | Lysing Solution |
| Unit Size | Each |
| Intended Use | For Research Use Only. Not for use in diagnostics. |
Invitrogen™ High-Yield Lyse offers a reliable, fixative-free solution for erythrocyte removal in whole blood processing. Optimized for post-staining lysis and flow cytometry, it ensures high yield, minimal cell loss, and cleaner gating for rare and sensitive leukocyte populations.