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The Human Interferon γ (IFN-γ) ELISA Kit is designed for the quantitative measurement of IFN-γ concentrations in serum, plasma, cell culture supernatants, and other biological fluids.
Interferon-γ is a critical cytokine secreted primarily by activated T cells and NK cells, playing a central role in immune regulation, inflammation, and antiviral responses.
This kit employs a high-sensitivity sandwich ELISA method, pre-coated with a monoclonal antibody specific for human IFN-γ, ensuring precise and reproducible quantification suitable for immunology and cell biology research.
Key Features
- High Sensitivity: Detection limit of 9.4 pg/mL with an assay range of 15.6–1,000 pg/mL.
- Precision Performance: Intra-assay CV < 10%, inter-assay CV < 12%.
- Comprehensive Kit: Includes standards, buffers, HRP conjugate, and substrate reagents for 96 determinations.
- Rapid Assay Time: Total time approximately 3 hours.
- Reliable Quantification: Linear standard curve with reproducible optical density readings at 450 nm.
- Validated Protocol: Based on the sandwich ELISA principle with HRP-TMB colorimetric detection.
- Quality Controlled: Manufactured under strict production and testing guidelines for consistent lot-to-lot reproducibility.
Principle of the Assay
This assay is based on a sandwich ELISA technique:
- The 96-well microplate is pre-coated with anti-human IFN-γ antibody.
- Samples and standards are added, allowing IFN-γ to bind the immobilized antibody.
- A HRP-conjugated detection antibody binds to the captured IFN-γ.
- Following washing, TMB substrate is added for color development.
- The reaction is terminated, and absorbance is measured at 450 nm, proportional to IFN-γ concentration.
- Concentrations are determined via comparison with a standard curve.
Specifications
| Parameter | Specification |
|---|---|
| Detection Method | Sandwich ELISA (Colorimetric) |
| Detection Range | 15.6 – 1,000 pg/mL |
| Sensitivity | 9.4 pg/mL |
| Precision | Intra-assay CV < 10%; Inter-assay CV < 12% |
| Standard Concentration | 1,080 pg/mL |
| Sample Types | Serum, plasma, cell culture supernatants, tissue extracts, biological fluids |
| Storage Conditions | 2–8 °C |
| Shelf Life | 6 months |
| Sample Volume | 10 µL per test |
| Detection Wavelength | 450 nm |
| Total Assay Time | ~3 hours |
| Intended Use | For Research Use Only (RUO) |
Kit Components (96 Tests)
| Component | Volume/Quantity | Storage |
|---|---|---|
| User Manual | 1 | Room Temp |
| Closure Plate Membrane | 2 | Room Temp |
| Microelisa Strip Plate (Pre-coated) | 1 × 96 wells | 2–8 °C |
| Standard (1,080 pg/mL) | 0.5 mL × 1 | 2–8 °C |
| Standard Diluent | 1.5 mL × 1 | 2–8 °C |
| HRP-Conjugate Reagent | 6 mL × 1 | 2–8 °C |
| Sample Diluent | 6 mL × 1 | 2–8 °C |
| Chromogen Solution A | 6 mL × 1 | 2–8 °C |
| Chromogen Solution B | 6 mL × 1 | 2–8 °C |
| Stop Solution | 6 mL × 1 | 2–8 °C |
| Wash Solution (30× concentrate) | 20 mL × 1 | 2–8 °C |
Applications
- Quantification of human IFN-γ in serum or plasma
- Cytokine profiling in immunological and inflammatory response research
- Cell culture supernatant analysis for cytokine secretion studies
- Immunotherapy and vaccine development research
- Autoimmune and infectious disease studies related to IFN-γ signaling pathways
Safety and Regulatory Notes
- For Research Use Only (RUO): Not for therapeutic, diagnostic, or clinical applications.
- Store all reagents at 2–8 °C and avoid repeated freeze–thaw cycles.
- Handle all human-derived materials as potentially infectious.
- Reagents from different lot numbers should not be mixed.
The Human Interferon γ (IFN-γ) ELISA Kit delivers sensitive, accurate, and reproducible detection of IFN-γ in a variety of biological samples.
With its robust performance, simple protocol, and high-quality reagents, it is an indispensable tool for immunological, biomedical, and cytokine research laboratories investigating cellular immune responses and inflammatory mechanisms.
No resources are currently available for this product.
The Human Interferon γ (IFN-γ) ELISA Kit is designed for the quantitative measurement of IFN-γ concentrations in serum, plasma, cell culture supernatants, and other biological fluids.
Interferon-γ is a critical cytokine secreted primarily by activated T cells and NK cells, playing a central role in immune regulation, inflammation, and antiviral responses.
This kit employs a high-sensitivity sandwich ELISA method, pre-coated with a monoclonal antibody specific for human IFN-γ, ensuring precise and reproducible quantification suitable for immunology and cell biology research.
Key Features
- High Sensitivity: Detection limit of 9.4 pg/mL with an assay range of 15.6–1,000 pg/mL.
- Precision Performance: Intra-assay CV < 10%, inter-assay CV < 12%.
- Comprehensive Kit: Includes standards, buffers, HRP conjugate, and substrate reagents for 96 determinations.
- Rapid Assay Time: Total time approximately 3 hours.
- Reliable Quantification: Linear standard curve with reproducible optical density readings at 450 nm.
- Validated Protocol: Based on the sandwich ELISA principle with HRP-TMB colorimetric detection.
- Quality Controlled: Manufactured under strict production and testing guidelines for consistent lot-to-lot reproducibility.
Principle of the Assay
This assay is based on a sandwich ELISA technique:
- The 96-well microplate is pre-coated with anti-human IFN-γ antibody.
- Samples and standards are added, allowing IFN-γ to bind the immobilized antibody.
- A HRP-conjugated detection antibody binds to the captured IFN-γ.
- Following washing, TMB substrate is added for color development.
- The reaction is terminated, and absorbance is measured at 450 nm, proportional to IFN-γ concentration.
- Concentrations are determined via comparison with a standard curve.
Specifications
| Parameter | Specification |
|---|---|
| Detection Method | Sandwich ELISA (Colorimetric) |
| Detection Range | 15.6 – 1,000 pg/mL |
| Sensitivity | 9.4 pg/mL |
| Precision | Intra-assay CV < 10%; Inter-assay CV < 12% |
| Standard Concentration | 1,080 pg/mL |
| Sample Types | Serum, plasma, cell culture supernatants, tissue extracts, biological fluids |
| Storage Conditions | 2–8 °C |
| Shelf Life | 6 months |
| Sample Volume | 10 µL per test |
| Detection Wavelength | 450 nm |
| Total Assay Time | ~3 hours |
| Intended Use | For Research Use Only (RUO) |
Kit Components (96 Tests)
| Component | Volume/Quantity | Storage |
|---|---|---|
| User Manual | 1 | Room Temp |
| Closure Plate Membrane | 2 | Room Temp |
| Microelisa Strip Plate (Pre-coated) | 1 × 96 wells | 2–8 °C |
| Standard (1,080 pg/mL) | 0.5 mL × 1 | 2–8 °C |
| Standard Diluent | 1.5 mL × 1 | 2–8 °C |
| HRP-Conjugate Reagent | 6 mL × 1 | 2–8 °C |
| Sample Diluent | 6 mL × 1 | 2–8 °C |
| Chromogen Solution A | 6 mL × 1 | 2–8 °C |
| Chromogen Solution B | 6 mL × 1 | 2–8 °C |
| Stop Solution | 6 mL × 1 | 2–8 °C |
| Wash Solution (30× concentrate) | 20 mL × 1 | 2–8 °C |
Applications
- Quantification of human IFN-γ in serum or plasma
- Cytokine profiling in immunological and inflammatory response research
- Cell culture supernatant analysis for cytokine secretion studies
- Immunotherapy and vaccine development research
- Autoimmune and infectious disease studies related to IFN-γ signaling pathways
Safety and Regulatory Notes
- For Research Use Only (RUO): Not for therapeutic, diagnostic, or clinical applications.
- Store all reagents at 2–8 °C and avoid repeated freeze–thaw cycles.
- Handle all human-derived materials as potentially infectious.
- Reagents from different lot numbers should not be mixed.
The Human Interferon γ (IFN-γ) ELISA Kit delivers sensitive, accurate, and reproducible detection of IFN-γ in a variety of biological samples.
With its robust performance, simple protocol, and high-quality reagents, it is an indispensable tool for immunological, biomedical, and cytokine research laboratories investigating cellular immune responses and inflammatory mechanisms.
No resources are currently available for this product.
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Specifications
| Pack Size | For 48 Tests, For 96 Tests |