Human Interferon α (IFN-α) ELISA Kit

The Human IFN-α ELISA Kit is a high-sensitivity enzyme immunoassay designed for the quantitative detection of human interferon alpha (IFN-α) in a wide range of biological samples. Utilizing the well-established enzyme-linked immunosorbent assay (ELISA) principle, this kit delivers reliable, reproducible, and accurate results for research applications in immunology, virology, oncology, and inflammatory disease studies.

Engineered for ease of use and consistent performance, the kit includes pre-coated microplates, optimized reagents, and a complete standard system, enabling efficient detection workflows in laboratory environments.

Key Features & Benefits

High Sensitivity & Accuracy

• Detection sensitivity as low as 0.5 pg/mL
• Broad assay range of 1.6 – 100 pg/mL
• Suitable for detecting low-abundance cytokines

Reliable & Reproducible Results

• Intra-assay CV: <10%
• Inter-assay CV: <12%
• Pre-validated reagents ensure consistency across experiments

Wide Sample Compatibility

• Compatible with:
  • Serum
  • Plasma
  • Cell culture supernatants
  • Tissue homogenates
  • Other biological fluids

Optimized ELISA Workflow

• Pre-coated antibody microplate for convenience
• HRP-conjugate detection system for strong signal generation
• Colorimetric readout at 450 nm for precise quantification

Flexible Kit Sizes

• Available in:
  • 48-test format (SL0957Hu-48T)
  • 96-test format (SL0957Hu-96T)

Principle of the Assay

This kit operates on a sandwich ELISA principle:

1. Microplate wells are coated with antibodies specific to human IFN-α
2. IFN-α in samples binds to immobilized antibodies
3. An HRP-labeled secondary antibody binds to the antigen
4. Addition of TMB substrate produces a blue color reaction
5. Stop solution converts the color to yellow
6. Absorbance is measured at 450 nm
7. Concentration is determined using a standard calibration curve

Applications

• Cytokine profiling and immune response studies
• Viral infection research (e.g., interferon response)
• Cancer and tumor immunology research
• Drug discovery and biomarker validation
• Inflammation and autoimmune disease studies

Technical Specifications

Storage & Stability

• Store all reagents at 2–8°C
• Maintain sealed plate strips with desiccant
• Avoid repeated freeze-thaw cycles of samples
• Prepared wash buffer stable for 30 days

Unique Advantages

• High sensitivity for low-level cytokine detection
• Complete kit system with all essential reagents included
• User-friendly protocol suitable for routine laboratory workflows
• Robust performance with minimal variability
• Cost-effective solution for research laboratories

The Human IFN-α ELISA Kit is a dependable and efficient solution for researchers requiring precise quantification of interferon alpha in biological samples. With its high sensitivity, reproducibility, and streamlined workflow, this kit is well-suited for advanced immunological and biomedical research applications.