Halt™ Protease Inhibitor Cocktail (100X)

The Thermo Scientific™ Halt™ Protease Inhibitor Cocktail (100X) is a ready-to-use, highly effective solution designed to protect proteins from proteolytic degradation during cell lysis and protein extraction. This broad-spectrum inhibitor cocktail prevents the activity of endogenous proteases, ensuring protein stability for downstream applications.

Key Features:

1. Ready-to-Use Format:
   • Convenient 100X liquid concentrate, requiring only 10 μL per 1 mL of lysis buffer.
   • Eliminates the need for preparing individual protease inhibitors.
2. Broad-Spectrum Protection:
   • Inhibits serine-proteases, cysteine-proteases, aspartic acid-proteases, and aminopeptidases.
   • Contains six optimized inhibitors:
     • AEBSF
     • Aprotinin
     • Bestatin
     • E-64
     • Leupeptin
     • Pepstatin A
3. Optional Metalloprotease Inhibition:
   • Includes a separate vial of EDTA solution for metalloprotease inhibition, providing flexibility for different workflows.
4. Refrigerator-Stable:
   • Stable at 4°C, ensuring long-term usability and eliminating the need for freezing.
5. Compatible with Various Lysis Methods:
   • Works seamlessly with Thermo Scientific Pierce Cell Lysis Buffers, sonication, French press, and other lysate preparation techniques.
6. Flexible Packaging Options:
   • Available in multiple sizes, including single-use microtubes, reducing waste and contamination risk.

Applications:

1. Protein Protection:
   • Prevents degradation of cellular proteins during lysis and extraction processes.
2. Protease Activity Screening:
   • Used to inhibit unwanted protease activity during lysate preparation.
3. Regulatory Studies:
   • Facilitates studies on the role of proteolysis in cellular regulation.
4. Broad Compatibility:
   • Suitable for use with commercial or homemade detergent-based lysis buffers.

Specifications:

Advantages:

1. Comprehensive Protection:
   • Effectively inhibits multiple classes of proteases, preserving protein integrity for downstream applications.
2. Convenience:
   • Ready-to-use liquid formulation simplifies workflows and reduces preparation errors.
3. Optional EDTA Inclusion:
   • Provides flexibility to inhibit metalloproteases when required.
4. Reproducible Results:
   • High-quality formulation ensures consistent performance across experiments.

Storage and Handling:

• Store at 4°C in its original container.
• Avoid repeated freeze/thaw cycles to maintain inhibitor stability.

Product Details:

Related Products:

• Halt™ Protease Inhibitor Single-Use Cocktail, EDTA-Free (100X)
• Thermo Scientific Pierce Cell Lysis Buffers

The Thermo Scientific™ Halt™ Protease Inhibitor Cocktail (100X) offers a comprehensive solution for preventing proteolytic degradation in protein samples. Its ease of use, broad-spectrum protection, and flexibility make it an essential reagent for protein research and cellular studies.