Gibco™ Viral Production Cells

The Gibco™ Viral Production Cells are a HEK 293F-derived cell line optimized for lentiviral vector production and serve as a core component of the LV-MAX™ Lentiviral Production System (Cat. No. A35684). These cells are adapted to chemically defined, serum-free, and protein-free LV-MAX™ Production Medium (A3583401), allowing for high-density suspension culture and scalable lentivirus production.

Compared to traditional polyethyleneimine (PEI)-based transfection methods, LV-MAX™ transfection reagent (A35348) enables higher transfection efficiency and superior lentiviral vector yields.

Key Features & Benefits

1. High-Yield Lentiviral Production

• Optimized for lentiviral vector generation in the LV-MAX™ system.
• Produces higher viral titers than conventional HEK 293 cell lines.
• Ideal for gene therapy research, CAR-T cell engineering, and viral vector manufacturing.

2. Scalable Suspension Culture for High-Throughput Production

• Adapted for serum-free, protein-free LV-MAX™ Production Medium.
• Supports scalability from 96-deep well blocks to 2-L flasks and bioreactors.
• High cell viability and rapid growth rates for efficient lentivirus packaging.

3. Ready-to-Use, Frozen Cell Format

• Supplied as frozen cells (1 × 10⁷ viable cells/mL) in LV-MAX™ Production Medium with 10% DMSO.
• Fast and efficient thawing directly into LV-MAX™ Production Medium.
• Requires a humidified CO₂ incubator with shaker platform for optimal growth.

Specifications

Available Product Variants

Applications

1. Lentiviral Vector Production for Gene Therapy & Research

• Optimized for high-yield lentivirus generation for gene delivery applications.
• Scalable transient lentivirus production in biotech and academic research.

2. Cell & Gene Therapy Development

• Supports CAR-T cell engineering and gene editing applications.
• Enables consistent and reproducible viral vector manufacturing.

3. Bioprocessing & Virus-Based Drug Discovery

• Designed for large-scale production of lentiviral vectors.
• Ideal for bioreactor-based scalable lentivirus generation.

Storage & Handling

• Store in liquid nitrogen until ready to use.
• Do not store at -80°C, as it may affect cell viability.
• Thaw rapidly in a 37°C water bath and immediately transfer to LV-MAX™ Production Medium.
• Maintain in shaking suspension culture for optimal growth and lentivirus production.

Ordering Information

The Gibco™ Viral Production Cells are a HEK 293F-derived suspension cell line optimized for high-yield lentiviral vector production within the LV-MAX™ Lentiviral Production System. These cells offer scalable growth in serum-free conditions, ensuring reproducible viral titers for gene therapy, bioprocessing, and CAR-T cell research.