Gibco™ OptiPRO™ SFM

Gibco™ OptiPRO™ Serum-Free Medium (SFM) is a chemically defined, serum-free, animal origin–free, low-protein culture medium designed for the growth of kidney-derived cell lines, including MDBK, MDCK, PK-15, and VERO, commonly used in virus production and recombinant protein expression. In addition, OptiPRO™ SFM supports the growth of other mammalian cell lines such as HeLa, BHK-21, and COS-7, offering a versatile solution for multiple culture needs.

With a protein concentration of <10 µg/mL, OptiPRO™ SFM simplifies downstream purification of recombinant proteins. Manufactured under cGMP conditions in a facility compliant with ISO 13485 and FDA-registered, this medium provides consistent quality and performance for research and further manufacturing applications.

Key Features

• Serum-Free and Animal Origin-Free – Reduces variability and eliminates animal-derived components.
• Low Protein Content (<10 µg/mL) – Facilitates efficient purification of recombinant proteins.
• Versatile Cell Line Compatibility – Optimized for MDBK, MDCK, PK-15, and VERO; validated for HeLa, BHK-21, COS-7, and more.
• Direct Adaptation – Enables smooth transition of many cell lines to serum-free conditions.
• Manufactured to cGMP Standards – Produced in an FDA-registered, ISO 13485–certified facility.

Applications

• Growth and maintenance of kidney-derived and other mammalian cell lines.
• Virus production (e.g., for vaccine development).
• Recombinant protein production requiring low-protein medium for downstream processing.
• Research and further manufacturing workflows requiring defined, serum-free formulations.

Technical Specifications

Unique Advantages

• Provides a defined, consistent formulation for reproducible cell culture.
• Simplifies downstream viral and protein purification workflows due to low protein content.
• Eliminates risks of contamination from serum or animal-derived components.
• A single medium solution for multiple mammalian cell lines, increasing convenience and cost-effectiveness.

The Gibco™ OptiPRO™ SFM is a versatile, serum-free, animal origin–free medium designed to support a broad range of mammalian cell lines, particularly kidney-derived lines widely used in virus and recombinant protein production. With its low-protein formulation, cGMP manufacturing, and ISO 13485 certification, it ensures consistency, reproducibility, and efficiency across research and manufacturing workflows.