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Widely Trusted Basal Medium for Robust Mammalian Cell Growth
Gibco™ Dulbecco’s Modified Eagle Medium (DMEM), High Glucose is a widely used, nutrient-rich basal medium formulated to support the growth of a broad range of mammalian cell types. This DMEM formulation contains 4.5 g/L glucose, L-glutamine, and phenol red, and is free of sodium pyruvate and HEPES, making it ideal for cell culture workflows that require defined supplementation and controlled conditions.
Originally developed as an enhancement to Eagle’s Minimal Essential Medium (MEM), DMEM offers four times the concentration of amino acids and vitamins, supporting enhanced cell growth, viability, and metabolic activity.
Key Features & Benefits
-
High Glucose Formulation (4.5 g/L)
Supports high-energy-demanding cell types and long-term cultures. -
L-Glutamine Included
Provides a readily available source of nitrogen to support protein and nucleotide biosynthesis. -
Phenol Red Indicator
Enables real-time monitoring of pH shifts in the medium. -
Animal-Origin Free & Sterile-Filtered
Manufactured under cGMP-compliant, ISO 13485-certified conditions to ensure consistency and safety. -
Versatile Application
Suitable for a wide variety of primary cells and immortalized cell lines, including HeLa, HEK293, Cos-7, PC-12, as well as neurons, fibroblasts, glial cells, HUVECs, and smooth muscle cells. -
Standard Supplementation Ready
Requires serum supplementation, commonly 10% Fetal Bovine Serum (FBS), as it contains no proteins or growth factors.
Recommended Applications
- Mammalian cell culture expansion
- Primary cell isolation and maintenance
- Transfection and expression studies
- Recombinant protein and viral production
- Drug screening and toxicity testing
Specifications Table
| Attribute | Details |
|---|---|
| Product Name | Gibco™ DMEM, High Glucose |
| Catalog Numbers | 11965084 (1000 mL), 11965092 (500 mL), 11965175 (10 L) |
| Glucose Concentration | 4500 mg/L (4.5 g/L) |
| With Additives | High Glucose, L-Glutamine, Phenol Red |
| Without Additives | Sodium Pyruvate, HEPES |
| Form | Liquid |
| Sterility | Sterile-filtered |
| Buffer System | Sodium bicarbonate (3.7 g/L); requires 5–10% CO₂ |
| Concentration | 1X |
| pH Indicator | Phenol Red |
| Storage Conditions | 2–8°C; protect from light |
| Shipping Conditions | Ambient |
| Shelf Life | 12 months from date of manufacture |
| Manufacturing Quality | cGMP-compliant under ISO 13485 |
| Regulatory Status | For Research Use or Further Manufacturing Only |
Usage Notes
- Supplementation: Add 10% FBS or other appropriate serum before use.
- CO₂ Environment: Maintain a 5–10% CO₂ atmosphere in the incubator to preserve physiological pH.
- Storage: Store at 2–8°C and protect from light. Do not freeze.
- Sterility: Use aseptic technique to maintain sterility during dispensing and culture.
Gibco™ DMEM, High Glucose is a foundational cell culture medium optimized to support robust mammalian cell proliferation and maintenance. Trusted globally for its consistency, quality, and compatibility with a wide range of cell types, this formulation provides researchers with a flexible and dependable solution for routine and advanced cell culture applications.
TFS-Assets_LSG_manuals_MAN0018898_DMEM_IFU.pdf
Widely Trusted Basal Medium for Robust Mammalian Cell Growth
Gibco™ Dulbecco’s Modified Eagle Medium (DMEM), High Glucose is a widely used, nutrient-rich basal medium formulated to support the growth of a broad range of mammalian cell types. This DMEM formulation contains 4.5 g/L glucose, L-glutamine, and phenol red, and is free of sodium pyruvate and HEPES, making it ideal for cell culture workflows that require defined supplementation and controlled conditions.
Originally developed as an enhancement to Eagle’s Minimal Essential Medium (MEM), DMEM offers four times the concentration of amino acids and vitamins, supporting enhanced cell growth, viability, and metabolic activity.
Key Features & Benefits
-
High Glucose Formulation (4.5 g/L)
Supports high-energy-demanding cell types and long-term cultures. -
L-Glutamine Included
Provides a readily available source of nitrogen to support protein and nucleotide biosynthesis. -
Phenol Red Indicator
Enables real-time monitoring of pH shifts in the medium. -
Animal-Origin Free & Sterile-Filtered
Manufactured under cGMP-compliant, ISO 13485-certified conditions to ensure consistency and safety. -
Versatile Application
Suitable for a wide variety of primary cells and immortalized cell lines, including HeLa, HEK293, Cos-7, PC-12, as well as neurons, fibroblasts, glial cells, HUVECs, and smooth muscle cells. -
Standard Supplementation Ready
Requires serum supplementation, commonly 10% Fetal Bovine Serum (FBS), as it contains no proteins or growth factors.
Recommended Applications
- Mammalian cell culture expansion
- Primary cell isolation and maintenance
- Transfection and expression studies
- Recombinant protein and viral production
- Drug screening and toxicity testing
Specifications Table
| Attribute | Details |
|---|---|
| Product Name | Gibco™ DMEM, High Glucose |
| Catalog Numbers | 11965084 (1000 mL), 11965092 (500 mL), 11965175 (10 L) |
| Glucose Concentration | 4500 mg/L (4.5 g/L) |
| With Additives | High Glucose, L-Glutamine, Phenol Red |
| Without Additives | Sodium Pyruvate, HEPES |
| Form | Liquid |
| Sterility | Sterile-filtered |
| Buffer System | Sodium bicarbonate (3.7 g/L); requires 5–10% CO₂ |
| Concentration | 1X |
| pH Indicator | Phenol Red |
| Storage Conditions | 2–8°C; protect from light |
| Shipping Conditions | Ambient |
| Shelf Life | 12 months from date of manufacture |
| Manufacturing Quality | cGMP-compliant under ISO 13485 |
| Regulatory Status | For Research Use or Further Manufacturing Only |
Usage Notes
- Supplementation: Add 10% FBS or other appropriate serum before use.
- CO₂ Environment: Maintain a 5–10% CO₂ atmosphere in the incubator to preserve physiological pH.
- Storage: Store at 2–8°C and protect from light. Do not freeze.
- Sterility: Use aseptic technique to maintain sterility during dispensing and culture.
Gibco™ DMEM, High Glucose is a foundational cell culture medium optimized to support robust mammalian cell proliferation and maintenance. Trusted globally for its consistency, quality, and compatibility with a wide range of cell types, this formulation provides researchers with a flexible and dependable solution for routine and advanced cell culture applications.
TFS-Assets_LSG_manuals_MAN0018898_DMEM_IFU.pdf
Specifications
| Pack Size | 10 Liters, 500 mL, 1 Liter |