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Gibco™ Human TPO (Thrombopoietin) Recombinant Protein
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Specifications:
| Application | Western Blot, ELISA-Based Assays, Functional Assay (FN) | ||
| Storage Temperature | -20°C | ||
| Product Type | Proteins & Peptides | Forms | lyophilized powder |
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Molecular Biology | ||
Gibco™ Human Thrombopoietin (TPO) Recombinant Protein is a high-purity cytokine essential for the proliferation and differentiation of megakaryocyte progenitor cells, leading to platelet production (thrombopoiesis). Expressed in HEK293 cells, this recombinant protein retains native glycosylation patterns, ensuring high bioactivity and stability for in vitro applications.
TPO plays a critical role in hematopoiesis, platelet production, and immune cell regulation, making it essential for megakaryocyte culture, stem cell research, leukemia studies, and drug screening assays.
Key Features & Benefits
- Highly Purified & Bioactive
- Purity >90% by SDS-PAGE ensuring minimal contaminants.
- ED50 = 0.5 - 5 ng/mL, promoting dose-dependent proliferation of human TF-1 cells.
- HEK293-Expressed for Native Glycosylation & Stability
- Provides more accurate biological activity compared to E. coli-derived recombinant proteins.
- Ensures enhanced protein stability for long-term experiments.
- Low Endotoxin for Consistency in Cell Culture
- Endotoxin concentration <1 EU/µg, reducing unwanted immune activation.
- Suitable for hematopoietic stem cell (HSC) expansion and differentiation assays.
- Lyophilized for Long-Term Storage & Easy Reconstitution
- Can be reconstituted in PBS containing 0.1% human serum albumin or BSA.
- Enables stable storage at -20°C to -80°C in working aliquots to prevent freeze-thaw degradation.
- Optimized for Functional Assays & Cell Proliferation Studies
- Stimulates megakaryocyte differentiation and platelet production.
- Supports bone marrow cell culture and leukemia research.
Product Specifications
| Feature | Details |
|---|---|
| Product Name | Gibco™ Human TPO (Thrombopoietin) Recombinant Protein |
| Catalog Numbers | PHC9514 (10 µg) |
| Expression System | HEK293 Cells |
| Molecular Weight | 85 kDa |
| Protein Class | Recombinant Cytokine |
| Purity | >90% by SDS-PAGE |
| Endotoxin Level | <1 EU/µg |
| Specific Activity | ED50 = 0.5 - 5 ng/mL (determined via TF-1 cell proliferation assay) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Storage Buffer | PBS (No Preservatives) |
| Storage Conditions | Lyophilized: -20°C, Reconstituted: -20°C to -80°C |
| Shipping Conditions | Dry Ice |
| Research Applications | Hematopoiesis, Megakaryocyte Differentiation, Stem Cell Research, Oncology |
Use Cases & Applications
1. Hematopoiesis & Megakaryocyte Differentiation
- Supports Platelet Formation & Bone Marrow Research
- Stimulates proliferation and differentiation of megakaryocyte progenitor cells.
- Used in ex vivo expansion of platelets for regenerative medicine and transfusion studies.
- Enhances Hematopoietic Stem Cell (HSC) Expansion
- Promotes differentiation of HSCs into megakaryocytes.
- Used in bone marrow culture systems.
2. Immunology & Inflammatory Disease Research
- TPO in Immune Modulation & Cytokine Research
- Plays a role in inflammatory signaling and immune cell regulation.
- Used in autoimmune disease studies and platelet dysfunction research.
- VEGF-Mediated Angiogenesis & Tumor Growth Studies
- Influences vascular growth and tumor microenvironment interactions.
- Helps in angiogenesis-related cancer research.
3. Cancer Research & Leukemia Studies
- Role in Myeloid Leukemia & Blood Cancers
- TPO signaling impacts leukemia cell proliferation and resistance mechanisms.
- Supports research on hematopoietic malignancies and targeted therapies.
- TPO in Drug Discovery & High-Throughput Screening
- Used in pharmaceutical research for hematological disorder treatments.
- Essential for screening small molecules affecting thrombopoiesis.
4. Clinical & Translational Research
- Thrombopoietin in Blood Clotting & Platelet Biology
- Used in studies related to thrombocytopenia, clotting disorders, and platelet dysfunction.
- Helps in understanding platelet response to hematological therapies.
- Biomarker Discovery & Personalized Medicine
- Used for cytokine profiling in hematologic disorders.
- Supports research on precision medicine and individualized treatments.
Recommended Reconstitution & Handling
Reconstitution Protocol:
- Centrifuge vial briefly before opening to bring lyophilized contents to the bottom.
- Reconstitute human TPO in PBS containing 0.1% human serum albumin or BSA.
- Aliquot & Store at -20°C to -80°C for extended stability.
- Avoid repeated freeze-thaw cycles to maintain protein activity.
Recommended Working Concentrations:
| Application | Concentration |
|---|---|
| Megakaryocyte Differentiation | 5–50 ng/mL |
| Platelet Expansion Studies | 10–100 ng/mL |
| Hematopoietic Stem Cell Expansion | 1–10 ng/mL |
| Myeloid Leukemia Proliferation Assays | 25–100 ng/mL |
Storage & Handling Instructions
- Lyophilized Storage: Store at -20°C in a desiccated environment.
- Reconstituted Storage: Store at ≤-20°C to -80°C in working aliquots.
- Avoid repeated freeze-thaw cycles to prevent degradation.
- Use aseptic techniques when handling and diluting the protein.
Why Choose Gibco™ Human TPO Recombinant Protein?
✅ High bioactivity (ED50 = 0.5 - 5 ng/mL) for megakaryocyte proliferation
✅ HEK293-expressed for superior stability and glycosylation
✅ Low endotoxin levels (<1 EU/µg) for reproducible cell culture studies
✅ Validated in functional assays including hematopoiesis and thrombopoiesis
✅ Versatile for immunology, stem cell, oncology, and drug discovery research
Gibco™ Human TPO Recombinant Protein is a critical growth factor for platelet formation, immune modulation, and hematopoietic research, ensuring high-performance results in functional and cell-based assays.
- Pack Size: 1mg 100 µg 10 µg