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GeneRuler™ 100 bp DNA Ladder, ready-to-use 6X TriTrack DNA Loading Dye
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Specifications:
| Application | Protein Electrophoresis & Western Blotting | ||
| Storage Temperature | 2-8°C | ||
| Product Type | DNA Ladders | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Molecular Biology | ||
The Thermo Scientific™ GeneRuler 100 bp DNA Ladder is a ready-to-load molecular weight marker designed for precise sizing and approximate quantification of double-stranded DNA fragments between 100 bp and 1,000 bp. Ideal for use on agarose and polyacrylamide gels, the ladder includes 10 distinct, chromatography-purified DNA fragments that produce sharp, evenly spaced bands for accurate gel interpretation.
The ladder is supplied pre-mixed with 6X TriTrack DNA Loading Dye, which contains Xylene Cyanol FF, Bromophenol Blue, and Orange G—three tracking dyes that allow visual monitoring of DNA migration during electrophoresis. This dye formulation simplifies gel loading and minimizes handling errors, improving workflow efficiency.
Key Features & Benefits
- ✅ Defined Size Range – Covers 100 bp to 1,000 bp, perfect for small PCR amplicon analysis.
- ✅ Ready-to-Use – Premixed with 6X TriTrack dye for direct gel loading.
- ✅ Sharp, Bright Bands – Individual chromatography-purified fragments provide high clarity.
- ✅ Triple-Color Tracking System – Xylene Cyanol FF, Bromophenol Blue, and Orange G enhance lane visibility.
- ✅ Quantitative Capability – DNA band intensity corresponds to defined DNA amounts.
- ✅ Room Temperature Stability – Stable for up to 6 months at RT; 24 months under storage conditions.
- ✅ Environmentally Friendly – Packaged in sustainable materials.
Applications
- Sizing of PCR products (100–1,000 bp)
- Quantification of short double-stranded DNA fragments
- DNA fragment analysis on agarose or polyacrylamide gels
- Molecular diagnostics research workflows
- Routine molecular biology teaching labs
Technical Specifications
| Parameter | Details |
|---|---|
| Size Range | 100 bp to 1,000 bp |
| Number of Fragments | 10 distinct bands |
| Concentration | Premixed with 6X TriTrack Loading Dye |
| Gel Compatibility | Agarose and polyacrylamide gels (1–2%) |
| Dyes Included | Xylene Cyanol FF, Bromophenol Blue, Orange G |
| Visualization | Ethidium Bromide, SYBR Safe |
| Shelf Life | 24 months (stored), 6 months (RT) |
| Packaging | Sustainable |
| Product Line | GeneRuler™ |
| Ready-to-Load | Yes |
Available Catalog Numbers
| Catalog Number | Format | Quantity |
|---|---|---|
| SM0244 | Ready-to-use | 5 × 50 µg (500 apps) |
| SM0243 | Ready-to-use | 50 µg (100 apps) |
The Thermo Scientific™ GeneRuler 100 bp DNA Ladder is an essential tool for electrophoresis-based sizing and quantification of short DNA fragments. Its ready-to-load format, sharp band resolution, and user-friendly triple-dye system make it ideal for applications ranging from routine PCR verification to high-resolution DNA fragment analysis. With its room-temperature stability and sustainable packaging, it offers both performance and convenience for researchers focused on accuracy and efficiency.
- Pack Size: 50 µg 5 x 50 µg