Invitrogen™ FluoReporter™ lacZ/Galactosidase Quantitation Kit

Sensitive fluorescence-based assay for β-galactosidase reporter gene detection

The Invitrogen™ FluoReporter™ lacZ/Galactosidase Quantitation Kit provides a high-sensitivity fluorescence-based method for detecting and quantifying β-galactosidase (lacZ) activity in biological samples. This reporter gene assay system uses 3-carboxyumbelliferyl β-D-galactopyranoside (CUG) as the substrate. Upon hydrolysis by β-galactosidase, CUG produces 7-hydroxycoumarin-3-carboxylic acid, a bright blue-fluorescent product.

This approach offers superior sensitivity compared to traditional colorimetric assays, making it suitable for applications where low-level lacZ expression must be accurately measured.

Key Features

• High Sensitivity – Detects β-galactosidase activity at low expression levels
• Fluorescent Readout – Produces a bright blue fluorescence for quantitative analysis
• Specific Detection – Optimized for lacZ reporter systems
• Flexible Use – Suitable for various cell types and assay formats
• Complete Kit – Contains all reagents needed for β-galactosidase quantitation

Applications

• Quantitative measurement of lacZ reporter gene expression
• Molecular cloning verification via lacZ activity
• Gene regulation and promoter activity studies
• Sensitive detection of β-galactosidase activity in cell lysates
• Dual-reporter assays in combination with other reporter systems

Specifications

The FluoReporter™ lacZ/Galactosidase Quantitation Kit is the ideal choice for researchers requiring highly sensitive, fluorescence-based β-galactosidase detection. Its specificity, low background, and quantitative capability make it suitable for advanced molecular biology and gene expression studies.