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The Invitrogen™ Glucose Colorimetric Detection Kit is a complete, ready-to-use assay for the quantitative measurement of glucose in a wide range of biological and research samples. Designed for colorimetric detection at 560 nm, this kit offers high sensitivity and accuracy, making it ideal for applications in metabolism research, enzymology, and cellular bioenergetics.
Key Features
- High Sensitivity – Detects glucose levels as low as 0.413 mg/dL.
- Broad Sample Compatibility – Works with serum, plasma, urine, buffers, and tissue culture media.
- Complete Kit – Includes 96-well plate(s), beta-D-glucose standard, assay buffer, and reagents.
- Rapid & Simple Workflow – Minimal hands-on time, results in 30 minutes.
- Species-Independent – Suitable for a wide range of biological samples.
Assay Principle
This assay is based on the enzymatic oxidation of glucose by glucose oxidase to produce hydrogen peroxide. In the presence of horseradish peroxidase (HRP), hydrogen peroxide reacts with a colorimetric substrate, forming a pink-colored product. The color intensity, measured at 560 nm using a microplate reader, is directly proportional to the glucose concentration.
A beta-D-glucose standard is provided to create a standard curve, ensuring precise quantification across the assay’s working range of 0.5–32 mg/dL.
Applications
- Metabolic Research – Monitor glucose levels in biological samples.
- Diabetes & Endocrinology Research – Evaluate glucose metabolism in experimental models.
- Bioenergetics Studies – Link glucose metabolism to ATP production and cellular health.
- Nutritional and Food Science – Assess glucose in food or beverage samples (research use only).
- High-Throughput Screening – Efficient for drug discovery and inhibitor studies.
Technical Specifications
| Parameter | Specification |
|---|---|
| Detection Method | Colorimetric (560 nm) |
| Sensitivity | 0.413 mg/dL |
| Standard Curve Range | 0.5–32 mg/dL |
| Sample Types | Serum, Plasma, Urine, Buffers, Tissue Culture Media |
| Reaction Time | 30 minutes |
| Enzymes Used | Glucose Oxidase, Horseradish Peroxidase |
| Assay Type | 96-well plate format |
| Kit Capacity | 2 × 96 tests |
| Storage | –20°C |
| Shipping Condition | Wet or Dry Ice |
| Species Reactivity | Species-independent |
Kit Contents
- Clear 96-well plate(s)
- Beta-D-glucose standard
- Assay buffer
- Colorimetric substrate
- Glucose oxidase
- Horseradish peroxidase (HRP)
- Technical datasheet
The Invitrogen™ Glucose Colorimetric Detection Kit provides a fast, sensitive, and reproducible method for quantifying glucose in a variety of sample types. With its ready-to-use format and robust enzymatic reaction, this kit is ideal for biological research, metabolic pathway studies, and high-throughput screening applications.
Product Information Sheet: Glucose Colorimetric Detection Kit
The Invitrogen™ Glucose Colorimetric Detection Kit is a complete, ready-to-use assay for the quantitative measurement of glucose in a wide range of biological and research samples. Designed for colorimetric detection at 560 nm, this kit offers high sensitivity and accuracy, making it ideal for applications in metabolism research, enzymology, and cellular bioenergetics.
Key Features
- High Sensitivity – Detects glucose levels as low as 0.413 mg/dL.
- Broad Sample Compatibility – Works with serum, plasma, urine, buffers, and tissue culture media.
- Complete Kit – Includes 96-well plate(s), beta-D-glucose standard, assay buffer, and reagents.
- Rapid & Simple Workflow – Minimal hands-on time, results in 30 minutes.
- Species-Independent – Suitable for a wide range of biological samples.
Assay Principle
This assay is based on the enzymatic oxidation of glucose by glucose oxidase to produce hydrogen peroxide. In the presence of horseradish peroxidase (HRP), hydrogen peroxide reacts with a colorimetric substrate, forming a pink-colored product. The color intensity, measured at 560 nm using a microplate reader, is directly proportional to the glucose concentration.
A beta-D-glucose standard is provided to create a standard curve, ensuring precise quantification across the assay’s working range of 0.5–32 mg/dL.
Applications
- Metabolic Research – Monitor glucose levels in biological samples.
- Diabetes & Endocrinology Research – Evaluate glucose metabolism in experimental models.
- Bioenergetics Studies – Link glucose metabolism to ATP production and cellular health.
- Nutritional and Food Science – Assess glucose in food or beverage samples (research use only).
- High-Throughput Screening – Efficient for drug discovery and inhibitor studies.
Technical Specifications
| Parameter | Specification |
|---|---|
| Detection Method | Colorimetric (560 nm) |
| Sensitivity | 0.413 mg/dL |
| Standard Curve Range | 0.5–32 mg/dL |
| Sample Types | Serum, Plasma, Urine, Buffers, Tissue Culture Media |
| Reaction Time | 30 minutes |
| Enzymes Used | Glucose Oxidase, Horseradish Peroxidase |
| Assay Type | 96-well plate format |
| Kit Capacity | 2 × 96 tests |
| Storage | –20°C |
| Shipping Condition | Wet or Dry Ice |
| Species Reactivity | Species-independent |
Kit Contents
- Clear 96-well plate(s)
- Beta-D-glucose standard
- Assay buffer
- Colorimetric substrate
- Glucose oxidase
- Horseradish peroxidase (HRP)
- Technical datasheet
The Invitrogen™ Glucose Colorimetric Detection Kit provides a fast, sensitive, and reproducible method for quantifying glucose in a variety of sample types. With its ready-to-use format and robust enzymatic reaction, this kit is ideal for biological research, metabolic pathway studies, and high-throughput screening applications.