Invitrogen™ Human IDO ELISA Kit

The Invitrogen™ Human IDO ELISA Kit is a robust, high-sensitivity sandwich ELISA designed to quantify human Indoleamine 2,3-Dioxygenase (IDO1) levels in biological samples, including plasma, serum, and supernatants. This assay is essential for researchers investigating the roles of IDO1 in various physiological and pathological processes, such as immunoregulation, cancer, infection, and inflammation.

Key Features:

1. High Sensitivity and Precision:
   • Analytical sensitivity: 0.81 ng/mL
   • Assay range: 0.819–200 ng/mL
   • Intra-assay CV: <10%
   • Inter-assay CV: <12%
2. Sample Compatibility:
   • Detects natural and recombinant human IDO1 in:
     • Plasma (50 µL)
     • Serum (50 µL)
     • Cell culture supernatants (50 µL)
3. Ease of Use:
   • Pre-coated 96-well plate for simplified workflow.
   • Requires only 1 hour and 20 minutes of hands-on time.
   • Total time-to-result: 4 hours and 45 minutes.
4. Robust and Reliable Components:
   • Includes biotinylated detection antibodies and HRP-conjugated SAV for sensitive detection.
   • Wash buffer, chromogen, stop solution, and adhesive plate covers ensure ease of execution.
5. For Research Use:
   • Not for diagnostic or therapeutic applications.

Kit Components:

• Pre-coated 96-well plate
• Standard for generating a calibration curve
• Biotinylated detection antibody
• Streptavidin-HRP conjugate
• Wash buffer and chromogen
• Stop solution
• Adhesive plate covers

Technical Specifications:

• Instrument compatibility: Requires a colorimetric microplate reader.
• Storage: 2–8°C (shipped on wet ice).
• Format: Sandwich ELISA.

Use Cases:

1. Immunoregulation and Tolerance Research:
   • Investigate IDO1’s role in suppressing immune responses by depleting tryptophan and producing kynurenines.
   • Study IDO1-mediated immune tolerance mechanisms in cancer and autoimmunity.
2. Cancer Biology:
   • Explore how tumor cells exploit IDO1 to create an immunosuppressive environment.
   • Measure IDO1 levels as a potential biomarker for tumor progression and immune escape.
3. Infectious Diseases:
   • Analyze IDO1 activity as a part of the immune response against viral, bacterial, or parasitic infections.
   • Understand how tryptophan depletion inhibits pathogen growth.
4. Pregnancy and Maternal-Fetal Tolerance:
   • Study IDO1’s role in promoting tolerance to the fetus during pregnancy.
   • Measure changes in IDO1 levels in pregnancy-related conditions.
5. Autoimmune Disorders:
   • Evaluate the involvement of IDO1 in modulating autoimmune responses.
   • Investigate potential therapeutic interventions targeting IDO1 pathways.
6. Inflammation and Cytokine Research:
   • Assess IDO1 induction by interferons, especially IFN-γ, in inflammatory conditions.
   • Use IDO1 as a marker for inflammation severity or therapeutic response.
7. Drug Development and Screening:
   • Screen compounds targeting IDO1 for potential therapeutic applications.
   • Measure IDO1 activity in preclinical studies involving immune-modulating agents.

Biological Context:

IDO1 is a heme-containing enzyme that plays a pivotal role in degrading tryptophan into kynurenine derivatives, affecting immune modulation, pathogen defense, and tumor progression. This enzyme is upregulated in inflammation, cancer, and pregnancy, making it a crucial target for understanding and intervening in these processes.

Advantages of the Invitrogen™ Human IDO ELISA Kit:

• High sensitivity and specificity for natural and recombinant IDO1.
• Versatile application in diverse sample types and research areas.
• Streamlined workflow suitable for high-throughput analysis.
• Reliable results supported by Invitrogen’s expertise in assay development.

The Invitrogen™ Human IDO ELISA Kit is an invaluable tool for advancing research in immunology, oncology, infectious diseases, and translational medicine.