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Invitrogen™ Alanine Aminotransferase (ALT/GPT) Activity Kit, 96 Tests
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Specifications:
| Application | Colorimetric Assay | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Assay Kits | Forms | Kit with Various components |
| Product Brand | Thermo Fisher Scientific™, Invitrogen | ||
| Product Grade | Molecular Biology | ||
The Invitrogen™ Alanine Aminotransferase (ALT/GPT) Activity Kit is a sensitive and reliable colorimetric assay designed for the quantitative determination of ALT activity in a variety of biological samples, including serum, plasma, tissue homogenates, cultured cells, and cell culture supernatants.
Alanine aminotransferase (ALT/GPT) is a pyridoxal phosphate-dependent enzyme involved in amino acid metabolism and is widely used as a clinical biomarker for liver function, including conditions such as liver cirrhosis, viral hepatitis, and alcoholic liver disease.
This assay is based on the enzymatic conversion of alanine and α-ketoglutarate to pyruvic acid and glutamic acid, followed by formation of a colored phenylhydrazone complex, which is quantified spectrophotometrically at 510 nm.
Key Features
- High sensitivity and accuracy for ALT activity detection
- Simple, user-friendly protocol with minimal hands-on time
- Rapid results with short incubation and total assay time
- Colorimetric detection compatible with standard microplate readers
- Suitable for a wide range of biological sample types
- Consistent performance with strict quality control standards
Applications
- Liver function studies and biomarker analysis
- Clinical and preclinical research
- Metabolic pathway investigations
- Drug toxicity and pharmacological studies
- Enzyme activity measurement in cell culture and tissue samples
Assay Principle
ALT catalyzes the conversion of:
- Alanine + α-ketoglutarate → Pyruvic acid + Glutamic acid
The generated pyruvic acid reacts with phenylhydrazine to form a reddish-brown phenylhydrazone complex under alkaline conditions. The intensity of the color, measured at 510 nm, is directly proportional to ALT activity in the sample.
Technical Specifications
| Parameter | Details |
|---|---|
| Detection Method | Colorimetric |
| Wavelength | 510 nm |
| Assay Range | 0.75 – 72.3 IU/L |
| Sensitivity | 0.75 IU/L |
| Sample Volume | 10 μL |
| Total Assay Time | ~80 minutes |
| Incubation Time | 50 minutes |
| Intra-assay CV | 5.3% |
| Inter-assay CV | 9% |
| Equipment Required | Microplate reader |
Sample Compatibility
- Serum
- Plasma
- Tissue homogenates
- Cultured cells
- Cell culture supernatants
Product Variant
| Catalog Number | Quantity |
|---|---|
| EEA001 | 96 tests |
Storage & Handling
- Store at 2–8°C
- Ship under dry ice or refrigerated conditions
- Avoid repeated freeze-thaw cycles for optimal performance
The Invitrogen™ ALT/GPT Activity Kit provides a robust and efficient solution for laboratories requiring accurate quantification of ALT enzyme activity. Its high sensitivity, ease of use, and reproducible performance make it ideal for both routine and advanced research applications.